PD-L1激活NF-κB促进子宫内膜癌细胞上皮间质转化的作用研究  

Role of PD-L1 on promoting epithelial-mesenchymal transition by activating NF-κB in endometrial cancer

作　　者：牛玉苗 黄浩 陈晓静[1] NIU Yumiao;HUANG Hao;CHEN Xiaojing(Zhejiang Provincial Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases,Women’s Hospital,School of Medicine,Zhejiang University,Hangzhou 310006,Zhejiang,China)

机构地区：[1]浙江大学医学院附属妇产科医院中心实验室、浙江省妇科重大疾病精准诊治研究重点实验室,浙江杭州310006

出　　处：《中国现代医生》2024年第8期59-63,共5页China Modern Doctor

摘　　要：目的探讨程序性死亡蛋白配体-1(programmed death ligand-1,PD-L1)过表达对子宫内膜癌细胞Ishikawa上皮间质转化的影响及可能机制。方法使用实验室构建的PD-L1过表达稳转细胞株Ishikawa/PD-L1及稳转空载对照组Ishikawa/EV,qPCR及Western blot法检测PD-L1过表达效率,Transwell和划痕实验检测细胞迁移能力和侵袭活性,Western blot法检测上皮间质转化相关蛋白表达和核转录因子-κB(nuclear factor kappa-B,NF-κB)磷酸化水平。结果与对照组相比,Ishikawa/PD-L1迁移能力与侵袭活性显著增强,Vimentin、N-cadherin、p-p65蛋白表达明显升高,E-cadherin表达明显下调。结论PD-L1可通过诱导NF-κB信号通路激活,促进上皮间质转化,进而增强子宫内膜癌细胞的迁移、侵袭能力。Objective To investigate the effect of programmed death ligand-1(PD-L1)overexpression on epithelial mesenchymal transformation(EMT)in endometrial cancer cells and its possible mechanism.Methods Ishikawa/PD-L1,a PD-L1 stable overexpression cell line constructed in the laboratory and the control Ishikawa/EV were used.The efficiency of PD-L1 overexpression was detected by qPCR and Western blot assay.The cell migration ability and invasion activity were detected by scratch assay and Transwell assay.The EMT-related protein and the phosphorylation level of nuclear transcription factor-κB(NF-κB)were detected by Western blot.Results Compared with the control group,the migration ability and invasion activity of Ishikawa/PD-L1 were significantly enhanced,and the expression of E-cadherin was significantly down-regulated,whereas vimentin,N-cadherin and p-p65 were significantly increased.Conclusion PD-L1 can promote EMT by inducing the activation of NF-κB pathway,and thus enhance the migration and invasion ability of endometrial cancer cells.

关 键 词：子宫内膜癌 程序性死亡蛋白配体-1 核转录因子-ΚB 上皮间质转化 

分 类 号：R737[医药卫生—肿瘤]