一起由金黄色葡萄球菌感染引起的食物中毒疫情的病原检测  

Pathogenic detection of food poisoning caused by Staphylococcus aureus

作　　者：兰培利 戴蕾[1] 赵瑞臻[1] 卫少华 周鹏 陈彦哲[1] 程春荣[1] LAN Peili;DAI Lei;ZHAO Ruizhen;WEI Shaohua;ZHOU Peng;CHEN Yanzhe;CHENG Chunrong(Zhengzhou Center for Disease Control and Prevention,Zhengz hou 450007,China)

机构地区：[1]郑州市疾病预防控制中心,河南郑州450007

出　　处：《现代疾病预防控制》2024年第2期138-141,共4页MODERN DISEASE CONTROL AND PREVENTION

摘　　要：目的对郑州市航空港区某饭店一起食物中毒疫情的相关样品进行实验室检测分析,为疫情处置提供科学依据。方法用细菌学诊断法对采集的可疑食物、病人呕吐物和排泄物等15份样品进行常见14种致病菌分离鉴定;对分离到的金黄色葡萄球菌菌株进行相应的特异性鉴别,并进行肠毒素毒力基因检测和药物敏感性实验,并采用脉冲场凝胶电泳(PFGE)法对菌株进行分子分型,分析菌株之间的相关性。结果从采集的15份样品中检出金黄色葡萄球菌6株,其中,食物中检出4株、呕吐物中检出1株、大便中检出1株。肠毒素毒力基因检测结果显示,该6株菌均携带sea基因。药敏结果显示,该6株菌均对青霉素耐药。脉冲场凝胶电泳技术分型结果显示,6株金黄色葡萄球菌的PFGE条带相似度93.10%,为同一克隆系。结论引起本次食物中毒的致病菌为来自于同一克隆系的金黄色葡萄球菌。Objective To analyze the samples related to a food poisoning occurring in a restaurant in the district of Zhengzhou Airport Economy Zone,and provide scientific evidence for food poisoning management.Methods Using bacteriological diagnostic methods,14 common pathogens were isolated and identified from samples of suspect food,patient vomit and feces.The isolated Staphylococcus aureus was identified by specific identification,and further detection of enterotoxin toxicity gene and vitro drug sensitivity were performed.The molecular typing of the strains was performed by pulsed field gel electrophoresis(PFGE)and the correlation among the strains were analyzed.Results Six strains of Staphylococcus aureus were found in the samples,4 strains in food,1 strain in vomit and 1 strain in feces.Enterotoxin gene detection results showed that the six strains all carried sea genes;drug resistant test results showed that the six strains were all resistant to penicillin.The results of PFGE showed that the six strains of Staphylococcus aureus had 93.10%homology,showing the same clone.Conclusions The pathogenic bacteria of this food poisoning was Staphylococcus aureus of the same clone.

关 键 词：金黄色葡萄球菌 食物中毒 肠毒素 耐药性 脉冲场凝胶电泳 

分 类 号：R595.7[医药卫生—内科学]