基于非靶代谢组学的糙皮侧耳子实体发育的关联物质及主要代谢通路分析  

Analyses of components and main pathways involved in fruiting body development of Pleurotus ostreatus based on untargeted metabolomics

作　　者：刘芹[1] 师子文 吴杰 黄保[1] 梁雪迪 崔筱[1] 胡素娟[1] 牛森园 孔维丽[1] LIU Qin;SHI Ziwen;WU Jie;HUANG Bao;LIANG Xuedi;CUI Xiao;HU Sujuan;NIU Senyuan;KONG Weili(Key Laboratory of Evaluation and Utilization of Germplasm Resources of Edible Fungi in Huang-Huai-Hai Region,Ministry of Agriculture and Rural Affairs,Institute of Edible Fungi,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China;College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,Henan,China;School of Life Sciences,Henan Institute of Science and Technology,Xinxiang 453003,Henan,China)

机构地区：[1]河南省农业科学院食用菌研究所农业农村部黄淮海地区食用菌种质资源鉴定和利用重点实验室,河南郑州450002 [2]河南农业大学生命科学学院,河南郑州450002 [3]河南科技学院生命科学学院,河南新乡453003

出　　处：《菌物学报》2024年第2期56-70,共15页Mycosystema

基　　金：河南省现代农业产业技术体系(HARS-22-08-S,HARS-22-08G1);河南省农业科学院基础性科研工作项目(2023JC17)。

摘　　要：本研究采用代谢组学方法鉴定并分析了糙皮侧耳原基和刚分化的子实体中的小分子代谢物质,以期解析糙皮侧耳子实体形成和发育的潜在机制。糙皮侧耳原基和子实体中共鉴定出545种代谢物,包含酚酸、脂类、氨基酸及其衍生物、核苷酸及其衍生物、有机酸、生物碱、单宁、木脂素和香豆素以及其他代谢物共9类。主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA)表明糙皮侧耳原基和子实体中的代谢物质具有显著差异。以VIP≥1、fold change≥2或≤0.5为条件,共筛选到253种差异代谢物。调控通路分析结果显示差异物质涉及76条代谢途径,推测该菌通过色氨酸代谢,苯丙氨酸、酪氨酸和色氨酸的生物合成,赖氨酸降解,D-精氨酸和D-鸟氨酸代谢,生物素代谢,莨菪烷、哌啶和吡啶生物碱的生物合成,酪氨酸代谢,嘌呤代谢,丙酸代谢和异喹啉生物碱的生物合成等主要代谢通路完成物质的深度转化及调控。本研究为糙皮侧耳子实体发育机制的研究提供了一定的理论依据。In order to explore the underlying mechanism of fruiting body formation and development of Pleurotus ostreatus,the small molecular metabolites in primordia and newly differentiated fruiting bodies were investigated through metabolomics approaches.All the 545 metabolites including phenolic acids,lipids,amino acids and their derivatives,nucleotides and their derivatives,organic acids,alkaloids,tannins,lignans and coumarins,and others were identified.Principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS‐DA)revealed a significant difference between metabolites of primordia and newly differentiated fruiting bodies.A total of 253 significantly differential metabolites was screened and identified under the conditions of VIP(varible importance in the projection)≥1 and fold change≥2 or≤0.5,involving in 76 pathways.It was inferred that the deep transformation and regulation of components were accomplished via tryptophan metabolism,phenylalanine,tyrosine and tryptophan biosynthesis,lysine degradation,D-arginine and D-ornithine metabolism,biotin metabolism,tropane,piperidine and pyridine alkaloid biosynthesis,tyrosine metabolism,purine metabolism,propanoate metabolism and isoquinoline alkaloid biosynthesis pathways.This study will provide a theoretical basis for exploring the developmental mechanism of Pleurotus ostreatus fruiting body.

关 键 词：糙皮侧耳 子实体发育 代谢组学 差异代谢物 代谢通路 

分 类 号：S646.14[农业科学—蔬菜学]