东亚木层孔菌与两种野生桑黄的活性成分及其抗氧化活性比较  

A comparative study on the bioactive components and antioxidant activities of Phellinus orientoasiaticus and two species of Sanghuangporus

作　　者：张思瑶 余盛武 魏玉莲[1] ZHANG Siyao;YU Shengwu;WEI Yulian(Key Laboratory of Forest Ecology and Management,Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,Liaoning,China;University of Chinese Academy of Sciences,Beijing 100049,China;Longquan Protection Center of Qianjiangyuan-Baishanzu National Park,Longquan 323700,Zhejiang,China)

机构地区：[1]中国科学院沈阳应用生态研究所、中国科学院森林生态与管理重点实验室,辽宁沈阳110016 [2]中国科学院大学,北京100049 [3]钱江源-百山祖国家公园龙泉保护中心,浙江龙泉323700

出　　处：《菌物学报》2024年第2期71-86,共16页Mycosystema

基　　金：国家自然科学基金(32270017);辽宁省应用基础研究计划(2022JH2/101300182)。

摘　　要：桑黄是一类著名的药用真菌,多糖、黄酮、三萜是桑黄主要的活性成分,三者的药用价值受到了广泛关注。东亚木层孔菌Phellinus orientoasiaticus是生长于蔷薇科活立木上的大型真菌,其外观形态与桑黄高度形似,但其药用价值的研究鲜有报道。本研究以东北地区研究广泛的鲍姆桑黄Sanghuangporusbaumii和忍冬桑黄S.lonicericola野生子实体为对比研究材料,以粗多糖、总黄酮、总三萜的含量及其抗氧化活性为对比研究指标,探究东亚木层孔菌的药用价值。鲍姆桑黄粗多糖含量为4.64 mg/g、忍冬桑黄为2.94 mg/g、东亚木层孔菌为3.68 mg/g;鲍姆桑黄的总黄酮含量为65.53mg/g、忍冬桑黄为187.74mg/g、东亚木层孔菌为46.19mg/g;鲍姆桑黄总三萜含量为5.38 mg/g、忍冬桑黄为15.87mg/g、东亚木层孔菌为10.17mg/g。通过HPLC分析3种子实体黄酮成分,发现鲍姆桑黄总黄酮中可能含有芹菜素和槲皮素这2种成分;忍冬桑黄总黄酮中可能含有芹菜素、柚皮素这2种成分;东亚木层孔菌总黄酮中可能含有芹菜素和芦丁这2种成分。比较3种真菌子实体黄酮和多糖的DPPH自由基清除能力、ABTS自由基清除能力、超氧阴离子清除能力和羟自由基清除能力等抗氧化活性,结果发现忍冬桑黄粗多糖和东亚木层孔菌粗多糖抗氧化活性较好,鲍姆桑黄总黄酮抗氧化活性较好,忍冬桑黄和东亚木层孔菌总三萜抗氧化活性较好。本研究显示,东亚木层孔菌的粗多糖、总黄酮和总三萜含量均比较高,抗氧化活性较好,具有良好的药用应用前景。The Sanghuangporus species are well-known due to their medicinal values.Polysaccharides,flavonoids and triterpenes are the main active ingredients of Sanghuangporus,and the medicinal values of the three have received widespread attention.Phellinus orientoasiaticus is a wood-inhabiting fungus growing in the living trees of Rosaceae.It is phylogenetically related with Sanghuangporus and its medicinal value is rarely reported.To determine the medicinal value of P.orientoasiaticus,the natural basidiomata of Sanghuangporus baumii and S.lonicericola from Northeast China were selected as comparative studying materials for they are extensively investigated medicinal fungal species.The content of crude polysaccharides,total flavonoids,and total triterpenoids and their antioxidant activities were used as comparative research indicators.The determination results showed that the polysaccharide content for S.baumii was 4.64 mg/g,2.94 mg/g for S.lonicericola,and 3.68 mg/g for P.orientoasiaticus.The total flavonoid content was 65.53 mg/g,187.74 mg/g,and 46.19 mg/g respectively.The total triterpene content of S.baumii was 5.38 mg/g,of S.lonicericola 15.87 mg/g,and of P.orientoasiaticus 10.17 mg/g.The antioxidant activities included DPPH free radical scavenging capacity,ABTS free radical scavenging ability,superoxide anion scavenging ability,and hydroxyl radical scavenging ability.The polysaccharides of S.lonicericola and P.orientoasiaticus showed comparatively high antioxidant activity,and the antioxidant activity of total triterpenoids of S.lonicericola and P.orientoasiaticus was also comparatively strong.This study demonstrates that P.orientoasiaticus has a good application prospect for its comparatively high polysaccharide,total flavonoid and total triterpenes content and excellent antioxidant activity.

关 键 词：药用真菌 锈革孔菌科 代谢产物 成分分析 

分 类 号：S567.3[农业科学—中草药栽培]