富硒黑木耳菌丝体硒多糖的提取与抗氧化活性  

Extraction and antioxidant activities of selenium polysaccharide from selenium-enriched Auricularia heimuer mycelia

作　　者：姜冬洋 苏林贺 陈亚东 张彦龙[1] 曾伟民[1] JIANG Dongyang;SU Linhe;CHEN Yadong;ZHANG Yanlong;ZENG Weimin(Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education/Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region/Heilongjiang Provincial Key Laboratory of Microbiology for College/School of Life Sciences,Heilongjiang University,Harbin 150080,Heilongjing,China)

机构地区：[1]黑龙江大学生命科学学院、农业微生物技术教育部工程研究中心、黑龙江省寒地生态修复与资源利用重点实验室、黑龙江省普通高校微生物重点实验室,黑龙江哈尔滨150080

出　　处：《菌物学报》2024年第2期112-125,共14页Mycosystema

基　　金：“一带一路”创新人才交流外国专家项目(G2021055)。

摘　　要：为了获得富硒黑木耳菌丝体硒多糖的最佳制备工艺并进一步评估抗氧化作用,本研究以黑木耳菌种九丰、黑丰和西藏6号作为原料,采用耐硒及梯度硒浓度富硒筛选、单因素试验、响应面试验设计分析及抗氧化测定的方法对富硒黑木耳筛选、硒多糖的提取优化和抗氧化活性进行研究。结果表明,3个黑木耳菌种中,九丰耐硒能力最强;富硒发酵实验中,黑木耳菌丝体发酵总硒含量在硒浓度为60μg/mL时最大;富硒黑木耳菌丝体硒多糖提取的最佳条件为:超声时间25.4 min,水浴时间56 min,料液比1:49(质量体积比),此条件下硒多糖提取率为(17.49±0.10)%。富硒黑木耳菌丝多糖对DPPH、羟基自由基具有一定的清除能力,并在总还原力试验中表现出较强的还原能力。本试验筛选出最优菌株及最佳富硒发酵硒浓度后,运用响应面的方法对富硒黑木耳菌丝体硒多糖提取条件进行优化,系统地测定出最佳提取条件。研究结果为黑木耳菌株富硒培养及黑木耳硒多糖高效提取及其功能挖掘提供了系统科学的理论依据。In order to obtain the best preparation process of selenium polysaccharide from selenium-enriched Auricularia heimuer mycelium,A.heimuer strains Jiufeng,Heifeng and Xizang 6 were used as materials,and selenium tolerance and gradient concentration selenium enrichment screening,single factor test,response interview design analysis,and antioxidant determination were carried out for extraction and process-optimization of selenium polysaccharide from the mycelia.Antioxidant determination method was used to examine antioxidant activity of selenium polysaccharide.The results showed that‘Jiufeng’had the strongest selenium tolerance among the three A.heimuer strains.Selenium enriched fermentation experiment indicated that the total selenium content of A.heimuer mycelia was the highest when the selenium concentration was 60μg/mL.The optimal conditions for selenium polysaccharide extraction were:ultrasonic time of 25.4 min,water bath time of 56 min,and material to liquid ratio of 1:49(g:mL).Under this condition,the extraction rate of selenium polysaccharide was(17.49±0.10)%.Selenium-enriched A.heimuer mycelial polysaccharide has certain scavenging ability to DPPH and hydroxyl radicals,and shows strong reducing ability in the total reducing power test.The research results provide a preliminary theoretical basis for the selenium enriched culture of A.heimuer strains,and extraction of selenium polysaccharides from A.heimuer mycelia.

关 键 词：黑木耳 硒多糖 响应面优化 抗氧化 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]