固定正畸矫治80例病人唾液基质金属蛋白酶8和基质金属蛋白酶9的水平变化  

Changes of saliva MMP-8 and MMP-9 levels in fixed orthodontic patients

作　　者：张婉君 孔中楠 马永平[1] 崔敬雅 杨茜 马文盛 WANG Hui;DING Weijie;LU Hongmei;LI Xiaoyu;HE Peng;ZHU Xiaoqin(Department of Preventive Health Care,Affiliated Hospital of Yangzhou University Huai'an Maternal and Child Health Care Center,Huai'an,Jiangsu 223001,China;School of Public Health,Xuzhou Medical University,Xuzhou,Jiangsu 221009,China)

机构地区：[1]保定市第二医院口腔科,河北保定071051

出　　处：《安徽医药》2024年第4期746-750,I0003,共6页Anhui Medical and Pharmaceutical Journal

基　　金：河北省科技计划项目(212777154)。

摘　　要：目的 探究固定正畸矫治病人唾液基质金属蛋白酶8(MMP-8)、基质金属蛋白酶9(MMP-9)水平的变化及临床意义。方法 选取2019年6月至2021年12月于保定市第二医院接受固定正畸矫治成年病人80例及健康受试者40例为研究对象。对病人固定矫治前(T1)、固定矫治1周(T2)、固定矫治1个月(T3)及健康受试者探诊出血(BOP)、菌斑指数(PLI)进行检测;收集健康受试者及病人T1、T2、T3时期唾液,采用ELISA检测唾液中MMP-8、MMP-9水平;流式细胞术对唾液白细胞含量进行检测;分别对MMP-8、MMP-9与牙龈BOP相关性进行分析;分离唾液白细胞,检测白细胞MMP-8、MMP-9表达水平变化。结果T2、T3时病人BOP[(21.36±8.79)%、(13.06±5.80)%]、PLI[(2.53±0.43)分、(1.89±0.39)分]及唾液中MMP-8[(0.43±0.11)μg/L、(0.32±0.10)μg/L]、MMP-9[(3.64±0.76)μg/L、(2.02±0.50)μg/L]、白细胞水平[(17 893.71±505.49)个、(8 532.18±421.89)个],显著高于T1水平[(5.05±2.11)%、(0.71±0.25)分、(0.16±0.08)μg/L、(0.25±0.13)μg/L、(2 308.66±178.04)个,P<0.05];唾液MMP-8、MMP-9水平与牙龈BOP、PLI呈正相关;进一步发现T2、T3时白细胞MMP-8、MMP-9表达水平显著高于T1水平(P<0.05)。受试者操作特征(ROC)曲线显示唾液MMP-8及MMP-9水平能较好预测牙龈炎程度。结论 正畸病人唾液MMP-8、MMP-9水平与牙龈炎病情呈正相关,唾液MMP-8、MMP-9可作为评估正畸病人牙龈炎的潜在生物标志物。Objective To explore the changes and clinical significance of saliva matrix metalloproteinases 8(MMP-8)and matrix metalloproteinases 9(MMP-9)levels in fixed orthodontic patients.Methods A total of 80 adult patients and 40 healthy subjects who received fixed corrective treatment in The Second Hospital of Baoding City from June 2019 to December 2021 were selected as the study subjects.The bleeding on probing(BOP)and plaque index(PLI)of patients before fixed orthodontic treatment(T1),1 week after treatment(T2),1 month after treatment(T3)and healthy subjects were detected.Saliva of healthy subjects and patients at T1,T2 and T3 were collected,and the levels of MMP-8 and MMP-9 in saliva were detected by ELISA.Flow cytometry was performed to detect leukocyte concentration in saliva.The correlations between MMP-8,MMP-9 and gingival BOP were analyzed,respectively.White blood cells in saliva were isolated,and the expression levels of MMP-8 and MMP-9 in leukocytes were detected.Results At T2 and T3,the levels of BOP[(21.36±8.79)%,(13.06±5.80)%],PLI[(2.53±0.43)points,(1.89±0.39)points]and saliva MMP-8[(0.43±0.11)μg/L,(0.32±0.10)μg/L],MMP-9[(3.64±0.76)μg/L,(2.02±0.50)μg/L],leukocyte counts[(17893.71±505.49),(8532.18±421.89)]were significantly higher than those at T1[(5.05±2.11)%,(0.71±0.25)points,(0.16±0.08)μg/L,(0.25±0.13)μg/L,(2308.66±178.04),P<0.05].Salivary MMP-8 and MMP-9 levels were positively correlated with gingival BOP and PLI.It was further found that the expression levels of MMP-8 and MMP-9 in leukocytes at T2 and T3 were significantly higher than those at T1(P<0.05).Receiver operating characteristic(ROC)curve showed that salivary MMP-8 and MMP-9 levels could better predict the degree of gingivitis.Conclusion The levels of MMP-8 and MMP-9 in saliva are positively correlated with the condition of gingivitis in orthodontic patients,and MMP-8,MMP-9 in saliva can be used as potential biomarkers to evaluate the gingivitis in orthodontic patients.

关 键 词：牙龈炎 正畸矫正器 基质金属蛋白酶8 基质金属蛋白酶9 牙菌斑指数 

分 类 号：R783.5[医药卫生—口腔医学]