YTH结构域家族蛋白2和叉头蛋白转录因子3在肺腺癌中的表达关系研究  

Expression relationship of YTHDF2 and FOXO3 in lung adenocarcinoma

作　　者：杨睿[1] 李占江 陈腾飞[1] YANG Rui;LI Zhanjiang;CHEN Tengfei(Department of Respiratory and Critical Care Medicine,The First Affiliated Hospital of Zhengzhou University,Zhengzhou,He'nan 450000,China)

机构地区：[1]郑州大学第一附属医院呼吸与危重症医学科,河南郑州450000

出　　处：《安徽医药》2024年第4期764-767,I0004,共5页Anhui Medical and Pharmaceutical Journal

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20190209)。

摘　　要：目的 分析YTH结构域家族蛋白2(YTHDF2)及叉头蛋白转录因子3(FOXO3)在肺腺癌病人中的表达及与预后的关系。方法 收集2020年1月至2021年5月在郑州大学第一附属医院行手术治疗的肺腺癌病人癌组织及对应的癌旁组织(距离癌组织5 cm以上)80对,收集病人临床病理资料;利用癌症基因组图谱(TCGA)数据库查询YTHDF2、FOXO3基因在肺腺癌中的表达水平;采用免疫组织化学法检测YTHDF2、FOXO3蛋白在肺腺癌组织中的表达情况;分析YTHDF2、FOXO3蛋白水平与肺腺癌病人临床病理资料的关系;分析肺腺癌中YTHDF2与FOXO3基因表达水平的相关性;对病人进行为期3年的随访,分析病人3年累积生存率。结果 YTHDF2、FOXO3蛋白在肺腺癌组织中的高表达率分别为34.00%、26.00%,明显低于癌旁正常组织中79.48%、61.54%(P<0.05)。YTHDF2蛋白表达情况与肺腺癌病人年龄、淋巴结转移和分化程度相关(P<0.05);与TNM分期、性别无关(P>0.05);FOXO3蛋白表达情况与肺腺癌病人性别和分化程度相关(P<0.05);肺腺癌组织中YTHDF2蛋白高表达组和低表达组病人3年累积生存率分别为53.30%、14.00%,经log-rank比较,差异有统计学意义(P<0.05);FOXO3蛋白高表达组和低表达组病人3年累积生存率分别为64.50%、12.20%,经Log-Rank比较,差异有统计学意义(P<0.05)。结论 YTHDF2、FOXO3在肺腺癌组织中均低表达,与病人肿瘤分化程度及3年累积生存率有关,有望成为评估肺腺癌病人预后的生物标志物。Objective To analyze the expressions of YTH domain family protein 2(YTHDF2)and forkhead transcription factor 3(FOXO3)in lung adenocarcinoma and their relationship with the prognosis of patients.Methods Eighty pairs of cancerous tissues and their corresponding normal adjacent tissues(more than 5 cm from the cancerous tissues)were collected from patients with lung adeno-carcinoma who underwent surgical treatment in The First Affiliated Hospital of Zhengzhou University from January 2020 to May 2021.The clinical pathological data of patients were collected.The Cancer Genome Atlas(TCGA)database was used to query the expression levels of YTHDF2 and FOXO3 genes in lung adenocarcinoma.Immunohistochemistry was applied to detect the expressions of YTHDF2 and FOXO3 proteins in lung adenocarcinoma tissues.The relationship between YTHDF2 and FOXO3 protein levels and clini-copathological data of patients with lung adenocarcinoma was analyzed,and the correlation between YTHDF2 and FOXO3 gene expres-sion levels in lung adenocarcinoma was analyzed.The patients were followed up for 3 years,and the 3-year cumulative survival rate of patients was analyzed.Results The high expression rates of YTHDF2 and FOXO3 proteins in lung adenocarcinoma tissues were 34.00%and 26.00%,which were significantly lower than 79.48%and 61.54%in normal adjacent tissues(P<0.05).The expression of YTHDF2 protein was correlated with the age,lymph node metastasis and differentiation degree of patients with lung adenocarcinoma(P<0.05),while was not correlated with TNM staging and gender(P>0.05).The expression of FOXO3 protein was correlated with the gen-der and degree of differentiation of patients with lung adenocarcinoma(P<0.05).The 3-year cumulative survival rates of patients in the high-expressed YTHDF2 protein group and low-expressed YTHDF2 protein group were 53.30%and 14.00%,respectively,and Log-Rank comparison results showed that the difference was statistically significant(P<0.05);the 3-year cumulative survival rates in the high-expressed FOXO3 protein

关 键 词：癌 非小细胞肺 肺腺癌 YTH结构域家族蛋白2 叉头蛋白转录因子3 临床病理特征 累积生存率 

分 类 号：R734.2[医药卫生—肿瘤]