非酒精性脂肪性肝病病人血清长链非编码RNA人类白细胞抗原复合体18水平与氧化应激指标、肝纤维化程度的相关性  

Correlation of serum lncRNA HCG18 level with oxidative stress indicators and liver fibrosis in patients with nonalcoholic fatty liver disease

作　　者：符鑫 刘悦[2] 彭鑫 黄婷婷 廖雪霞 张曼 FU Xin;LIU Yue;PENG Xin;HUANG Tingting;LIAO Xuexia;ZHANG Man(Department of Gastroenterology,Danzhou People's Hospital,Danzhou,Hainan 571799,China;Department of Digestive Oncology,Union Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei 430023,China)

机构地区：[1]儋州市人民医院消化内科,海南儋州571799 [2]华中科技大学同济医学院附属协和医院消化肿瘤外科,湖北武汉430023

出　　处：《安徽医药》2024年第4期768-772,共5页Anhui Medical and Pharmaceutical Journal

基　　金：湖北省自然科学基金项目(WJ2019A030)。

摘　　要：目的 探究非酒精性脂肪性肝病(NAFLD)病人血清长链非编码RNA人类白细胞抗原复合体18(lncRNA HCG18)与氧化应激指标及肝纤维化程度的相关性。方法 以儋州市人民医院2020年11月至2022年3月收治的92例NAFLD病人为NAFLD组,另选取同期健康体检志愿者90例为对照组。根据NAFLD肝纤维化(NFS)评分将NAFLD组病人分为排除晚期纤维化组(30例)、中间状态组(46例)、晚期纤维化组(16例)。采用实时荧光定量PCR(qRT-PCR)法检测血清lncRNA HCG18表达水平;酶联免疫吸附测定(ELISA)检测氧化应激指标水平;Pearson相关性分析NAFLD病人血清lncRNA HCG18与氧化应激指标的关系;Spearman相关性分析NAFLD病人血清lncRNA HCG18与NFS评分的关系。采用受试者操作特征(ROC)曲线分析血清lncRNA HCG18对NAFLD的诊断价值。结果 NAFLD组lncRNA HCG18(1.37±0.29比1.01±0.24)、丙二醛[(6.96±2.13)μmol/L比(4.17±1.04)μmol/L]水平明显高于对照组(P<0.001),谷胱甘肽过氧化物酶(GSH-Px)[(72.39±11.26)U/mL比(98.25±13.92)U/mL]、超氧化物歧化酶(SOD)[(68.36±10.65)U/mL比(92.84±12.89)U/mL]水平明显低于对照组(t=9.11、11.19、13.79、13.98,P<0.001)。排除晚期纤维化组、中间状态组、晚期纤维化组lncRNA HCG18(1.20±0.25比1.39±0.30比1.65±0.35)、丙二醛[(5.64±1.88)μmol/L比(7.12±2.16)μmol/L比(8.99±2.51)μmol/L]水平依次显著升高(F=12.36、13.06,P<0.001),GSH-Px[(81.56±13.58)U/mL比(71.74±10.52)U/mL比(57.06±9.05)U/mL]、SOD[(81.45±12.52)U/mL比(66.52±10.25)U/mL比(49.13±8.29)U/mL]水平依次显著降低(F=24.27、48.42,P<0.001)。Pearson相关性分析显示,NAFLD组血清lncRNA HCG18水平与丙二醛呈正相关(r=0.59,P<0.001),与GSH-Px、SOD均呈负相关(r=-0.57、-0.62,P<0.001);Spearman相关性分析显示NAFLD组血清lncRNA HCG18水平与NFS评分呈正相关(r=0.69,P<0.001)。血清lncRNA HCG18诊断NAFLD的ROC曲线下面积(AUC)为0.85。结论 NAFLD病人血清lncRNA HCG18显著升高,与氧化应激指标及肝�Objective To explore the correlation of serum long non-coding RNA human leukocyte antigen complex group 18(ln-cRNA HCG18)with oxidative stress indicators and the degree of liver fibrosis in patients with nonalcoholic fatty liver disease(NAFLD).Methods Ninety-two NAFLD patients admitted to Danzhou People's Hospital from November 2020 to March 2022 were chosen as the NAFLD group,and 90 healthy volunteers as the control group.According to the NAFLD liver fibrosis score(NFS),the patients in the NAFLD group were assigned into the exclusion of advanced fibrosis group(n=30),the intermediate state group(n=46),and the ad-vanced fibrosis group(n=16).The expression level of serum lncRNA HCG18 was detected by real-time fluorescence quantitative PCR(qRT-PCR),and the levels of oxidative stress indicators was detected by enzyme-linked immunosorbent assay(ELISA).Pearson correla-tion was used to analyze the relationship between serum lncRNA HCG18 and oxidative stress indicators in NAFLD patients,and Spear-man correlation was used to analyze the relationship between serum lncRNA HCG18 and NFS score in NAFLD patients.Receiver oper-ating characteristic(ROC)curve was used to analyze the diagnostic value of serum lncRNA HCG18 for NAFLD.Results The levels of lncRNA HCG18[(1.37±0.29)vs.(1.01±0.24)]and malondialdehyde(MDA)[(6.96±2.13)µmol/L vs.(4.17±1.04)µmol/L]in the NAFLD group were obviously higher than those in the control group,while the levels of glutathione peroxidase(GSH-Px)[(72.39±11.26)U/mL vs.(98.25±13.92)U/mL]and superoxide dismutase(SOD)[(68.36±10.65)U/mL vs.(92.84±12.89)U/mL]were obviously lower in the NAFLD group than those in the control group(t=9.11,11.19,13.79,13.98,P<0.001).The levels of lncRNA HCG18[(1.20±0.25)vs.(1.39±0.30)vs.(1.65±0.35)]and MDA[(5.64±1.88)µmol/L vs.(7.12±2.16)µmol/L vs.(8.99±2.51)µmol/L]in the exclusion of ad-vanced fibrosis group,the intermediate state group and the advanced fibrosis group were obviously increased in turn(F=12.36,13.06,P<0.001),while the levels of GSH-Px[(81.56±

关 键 词：脂肪肝 非酒精性脂肪性肝病 长链非编码RNA 人类白细胞抗原复合体18 氧化应激 肝纤维化 

分 类 号：R575.5[医药卫生—消化系统]