PTPRO通过减少STAT3磷酸化抑制黑色素瘤的恶性行为  

PTPRO Inhibits the Malignant Behavior of Melanoma by Reducing STAT3 Phosphorylation

作　　者：陈攀[1] 李彦 王佳慧 孔妙妮 舒佳辉 何梦淇 阮英[2] 佘同辉[1] CHEN Pan;LI Yan;SHE Tonghui(School of Basic Medical Sciences,Xianning Medical College,Hubei University of Science and Technology,Xianning Hubei 437100,China)

机构地区：[1]湖北科技学院医学部基础医学院,湖北咸宁437100 [2]湖北科技学院医学部临床医学院

出　　处：《湖北科技学院学报（医学版）》2024年第2期127-131,共5页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：国家大学生创新创业训练计划项目(202010927001);湖北省大学生创新创业训练计划项目(S202110927030)。

摘　　要：目的探讨酪氨酸磷酸酶O型变体(PTPRO)对黑色素瘤恶性行为的抑制作用及可能机制。方法应用针对人PTPRO基因启动子区的小激活RNA过表达黑色素瘤B16-F10细胞中的PTPRO,利用CCK8、平板克隆形成实验、划痕愈合实验、Trannswell小室迁移和侵袭实验分别检测激活PTPRO后黑色素瘤B16-F10细胞的存活、增殖、迁移和侵袭能力的改变。并用Western blot检测STAT3和磷酸化的STAT3的表达变化。结果PTPRO-saRNA组显著抑制黑色素瘤B16-F10细胞的活力(P<0.01)、克隆形成能力(P<0.01)、划痕愈合能力(P<0.01)、迁移和侵袭能力(P<0.01);而且,过表达PTPRO减少STAT3蛋白的磷酸化。结论过表达PTPRO通过减少STAT3的磷酸化抑制皮肤黑色素瘤的恶性行为。Objective To investigate the inhibition effect and possible mechanism of tyrosine phosphatase type O variant(PTPRO)on the malignant behavior of melanoma.Methods A small activated RNA targeting the promoter region of human PTPRO gene was used to overexpress PTPRO in melanoma B16-F10 cells.CCK8 assay,plate colony formation assay,scratch healing assay,Trannswell chamber migration and invasion assay were used to detect the changes of viability,proliferation,migration and invasion ability of melanoma B16-F10 cells after PTPRO overexpression.Western blot was used to detect the expression of STAT3 and phospho-STAT3.Results PTPRO-saRNA group significantly inhibited the viability(P<0.01),colony forming ability(P<0.01),scratch healing ability(P<0.01),migration(P<0.01)and invasion(P<0.01)of melanoma B16-F10 cells.Moreover,overexpression of PTPRO reduced the phosphorylation of STAT3 protein.Conclusion Overexpression of PTPRO can inhibit the malignant behavior of cutaneous melanoma by reducing STAT3 phosphorylation.

关 键 词：酪氨酸磷酸酶O型变体 黑色素瘤 磷酸化 STAT3 

分 类 号：R73[医药卫生—肿瘤]