离子色谱法测定中药材百合中的二氧化硫残留量  

Determination of Residual Sulfur Dioxide in Chinese Medicinal Lilium Brownii by Ion Chromatography

作　　者：邹沫君 ZOU Mo-jun(Leshan Center for Food and Drug Control,Leshan 614000,China)

机构地区：[1]乐山市食品药品检验检测中心,四川乐山614000

出　　处：《饮料工业》2024年第1期27-31,共5页Beverage Industry

摘　　要：目的:建立离子色谱/电导检测法测定百合中二氧化硫残留量的分析方法。方法:试样经酸蒸馏提取,离子色谱法测定硫酸根离子含量。选用IonPac AS19阴离子交换分离柱,在线产生40mmol/L KOH淋洗液等度洗脱,流速1.0mL/min,以抑制型电导检测器检测。结果:在0.0μg/mL~200.0μg/mL浓度范围内,线性关系良好,检出限为2.26mg/kg,定量限为7.53mg/kg,回收率为91.52%~98.65%(RSD值为0.89%~1.54%),精密度为0.40%~0.57%。结论:该方法操作简便、批量处理时间短、定性定量准确、灵敏度高,适合百合中二氧化硫残留量的检测。Object:A new method was established to determine residual sulfur dioxide in Lilium brownii by ion chromatography with conducting detection.Methods:Samples were extracted by acid distillation.The separation was performed on an Dionex IonPac AS19 anion analytical column.The mobile phase consisted of 40 mmol/L KOH solution in isocratic elution at a flow rate of 1.0mL/min.Auto-generated conducting detection ASRS ULTRA II 4mm was used.Results:The results showed that the linear relation of residual sulfur dioxide was good in the range of 0.0~200μg/mL(r=0.9998).The limits of detection of the method was 2.26mg/kg and the limit of quantitation was 7.53mg/kg.The average recoveries of residual sulfur dioxide in Lilium browniiranged between 91.52%~98.65%at different spiked levels with relative standard deviation from 0.89%to 1.54%.Precision was from 0.40 to 0.57%.Conclusion:The convenient operation and versatility of this method is suitable for the fast screening and detection of residual sulfur dioxide in Lilium brownii.

关 键 词：百合 二氧化硫 离子色谱 电导检测 

分 类 号：TS07[轻工技术与工程]