弥漫性大B细胞淋巴瘤患者增殖细胞核抗原、凋亡抑制蛋白Livin表达及意义  

Expression and significance of proliferating cell nuclear antigen and apoptosis inhibitory protein Livin in patients with diffuse large B-cell lymphoma

作　　者：刘冰[1] 王舒[1] 杨莹[1] 陈宛丽[1] 刘宁洒 Liu Bing;Wang Shu;Yang Ying;Chen Wanli;Liu Ningsa(Department of Hematology,Nanyang Second General Hospital,Nanyang 473000,China)

机构地区：[1]南阳市第二人民医院血液科,南阳473000

出　　处：《中国临床实用医学》2023年第6期1-6,共6页China Clinical Practical Medicine

基　　金：河南省医学科技攻关联合共建项目(LHGJ20215841)。

摘　　要：目的探讨弥漫性大B细胞淋巴瘤(DLBCL)患者组织中增殖细胞核抗原(PCNA)、凋亡抑制蛋白Livin表达与临床病理参数、预后的相关性。方法本研究为随机对照研究,选取2020年3月至2022年3月南阳市第二人民医院血液科收治的86例DLBCL患者,男46例,女40例,年龄(63.54±7.85)岁,年龄范围为47~80岁,于术中收集DLBCL组织、病灶旁正常淋巴组织标本。采用荧光定量聚合酶链式反应(qRT-PCR)、蛋白免疫印迹法分别检测组织中PCNA、Livin信使核糖核酸(mRNA)及蛋白水平。比较不同年龄、性别、肿瘤长径、累及淋巴结个数、Ann Arbor分期、骨髓侵犯、中枢神经侵犯、国际预后指数评分、美国东部肿瘤协作组(ECOG)评分、淋巴结转移患者PCNA、Livin mRNA水平,分析其与临床病理参数相关性。依据PCNA、Livin mRNA平均值分为高表达者、低表达者,比较不同表达者1年生存情况。采用Cox回归分析预后影响因素。结果DLBCL组织中PCNA mRNA(2.46±0.52)及蛋白水平(0.74±0.20)均高于正常淋巴组织[(1.00±0.08)、(0.26±0.05)],Livin mRNA(2.21±0.53)及蛋白水平(0.80±0.23)均高于正常淋巴组织[(1.01±0.07)、(0.33±0.09)],差异有统计学意义(P<0.05)。随着Ann Arbor分期增加,国际预后指数评分升高,ECOG评分升高,伴随骨髓侵犯、中枢神经侵犯、淋巴结转移,PCNA、Livin的mRNA水平均呈上升趋势,差异有统计学意义(P<0.05)。PCNA、Livin mRNA水平与Ann Arbor分期、骨髓侵犯、中枢神经侵犯、国际预后指数评分、ECOG评分、淋巴结转移呈正相关(P<0.05)。PCNA高表达者1年生存率[35.6%(16/45)]低于低表达者[82.9%(34/41)],Livin高表达者1年生存率[40.9%(18/44)]低于低表达者[71.4%(30/42)],差异有统计学意义(P<0.05)。Ann Arbor分期增加、淋巴结转移、PCNA mRNA及Livin mRNA水平升高为预后不良的独立危险因素(P<0.05)。结论PCNA、Livin表达水平升高与DLBCL患者临床病理参数密切相关,并可能作为评�Objective To investigate the expression of proliferating cell nuclear antigen(PCNA)and apoptosis suppressor protein Livin in tissues of patients with diffuse large B-cell lymphoma(DLBCL),and the correlation with clinicopathological parameters and prognosis.Methods This study was a randomized controlled study,a total of 86 DLBCL patients in the department of Hematology in Nanyang Second General Hospital from March 2020 to March 2022 were selected,including 46 males and 40 females,aged(63.54±7.85)years old,ranging from 47 to 80 years old.DLBCL tissue and normal lymphoid tissue samples were collected during the operation.Quantitative real-time polymerase chain reaction(qRT-PCR)and Western blot were used to detect PCNA and Livin mRNA and protein levels in tissues respectively.PCNA and Livin mRNA were compared among different age,sex,tumor diameter,number of lymph nodes involved,Ann Arbor stage,bone marrow invasion,central nervous invasion,international prognostic index score,Eastern Cooperative Oncology Group(ECOG)score,lymph node metastasis,and the correlation of PCNA and Livin mRNA with clinicopathological parameters were analyzed.According to the mean value of PCNA and Livin mRNA,they were divided into high expression group and low expression group,and the survival of different expression groups within 1 year was compared.Cox regression was used to analyze the prognostic factors.Results The PCNA mRNA(2.46±0.52)and protein levels(0.74±0.20)in DLBCL tissues were higher than those in normal lymphoid tissues[(1.00±0.08)and(0.26±0.05)],the mRNA(2.21±0.53)and protein levels(0.80±0.23)of Livin were higher than those of normal lymphoid tissue[(1.01±0.07)and(0.33±0.09)],the differences were statistically significant(P<0.05).With the increase of Ann Arbor stage,the international prognostic index score and ECOG score increased,and the mRNA levels of PCNA and Livin showed an increasing trend along with bone marrow invasion,central nervous invasion and lymph node metastasis,with statistical significance(P<0.05).PCNA a

关 键 词：弥漫性大B细胞淋巴瘤 增殖细胞核抗原 LIVIN 临床病理参数 

分 类 号：R733.1[医药卫生—肿瘤]