外用鱼油促进2型糖尿病大鼠伤口创面的愈合  

Topical fish oil accelerates wound healing in rats with type 2 diabetes mellitus

作　　者：黄荣江 辛文彬 倪向敏 李正宙 张贵明 王建 HUANG Rongjiang;XIN Wenbin;NI Xiangmin;LI Zhengzhou;ZHANG Guiming;WANG Jian(Department of Nutrition,Second Affiliated Hospital,Army Medical University(Third Military Medical University),Chongqing,400037,China)

机构地区：[1]陆军军医大学(第三军医大学)第二附属医院营养科,重庆400037

出　　处：《陆军军医大学学报》2024年第6期535-543,共9页Journal of Army Medical University

基　　金：重庆英才·创新创业领军人才项目:CQYC20220303514。

摘　　要：目的 观察局部外用鱼油对2型糖尿病(diabetes mellitus type 2, T2DM)大鼠伤口创面愈合的影响,并探讨其作用机制。方法 57只SD大鼠通过高脂高糖饮食联合STZ腹腔注射建立T2DM大鼠模型,建模成功49只。T2DM大鼠模型建立成功后通过外科方法创建伤口创面模型,采用随机数字表法分为鱼油组(n=17)、玉米油组(n=16)、模型对照组(n=16),各组于创面模型创建后次日开始给药,干预期间观察各组大鼠伤口创面愈合情况,分别于术后7、14 d取皮肤创面组织比较大鼠创面愈合情况,检测氧化应激指标(MDA、SOD、CAT)及炎症因子(TNF-α、IL-1β、IL-6、IL-10)水平,HE、Masson染色行病理学分析及观察皮肤真皮层胶原纤维沉积情况,Western blot检测SIRT1、Nrf2、NQO1蛋白表达。结果 与玉米油组和模型对照组相比,术后第7、14天鱼油组大鼠伤口创面愈合率明显提升(P<0.05),胶原纤维沉积增多;创面组织MDA、TNF-α、IL-1β、IL-6水平明显下降(P<0.05),而SOD、CAT、IL-10水平显著升高(P<0.05);Western blot结果显示,鱼油组大鼠创面组织SIRT1、Nrf2、NQO1蛋白表达水平较玉米油组和模型对照组显著提升(P<0.05);干预期间玉米油组与模型对照组间愈合效果无显著差异。结论 局部外用鱼油可有效促进T2DM大鼠伤口创面愈合,其机制可能与鱼油降低创面组织氧化应激及炎症反应有关。Objective To observe the effect of topical fish oil on wound healing in rats with type 2 diabetes mellitus(T2DM)and to investigate its mechanism of action.Methods The T2DM rat model was established on 57 SD rats with a high-fat,high-sugar diet combined with intraperitoneal injection of STZ,and finally,T2DM model was established successfully in 49 rats.Then wound model was inflicted surgically,and the rats were divided into fish oil group(n=17),corn oil group(n=16)and model control group(n=16).Topical medication was applied to each group on the next day after wound modelling.During the intervention period,the wound healing was observed in all rats of each group.After the wound tissue samples were collected on 7 and 14 d after wound modelling,the levels of oxidative stress indexes(MDA,SOD and CAT)and inflammatory factors(TNF-α,IL-1β,IL-6 and IL-10)were detected,and pathological changes and collagen fibril deposition in the dermis were observed with HE and Masson staining.The expression of SIRT1,Nrf2 and NQO1 was measured with Western blotting.Results Compared with the corn oil group and the model control group,the fish oil group had significantly improved wound healing rate on 7 and 14 d after modelling(P<0.05),increased collagen fibril deposition,decreased levels of MDA,TNF-α,IL-1βand IL-6 in wound tissue(P<0.05),while increased SOD,CAT and IL-10 levels(P<0.05),and protein levels of SIRT1,Nrf2 and NQO1 in the wound tissue(P<0.05).There were no statistical differences in above indicators between the corn oil group and the model control group(P<0.05).Conclusion Topical application of fish oil can effectively promote wound healing in T2DM rats,and the mechanism may be related to its reduction of oxidative stress and inflammation of wound.

关 键 词：鱼油 糖尿病伤口 氧化应激 炎症反应 沉默信息调节因子1(SIRT1) 

分 类 号：R587.2[医药卫生—内分泌] R641.05[医药卫生—内科学] R977.6[医药卫生—临床医学]