Effects of Tribulus terrestris saponins on proliferation and invasion of A549 cells  

作　　者：TANG Huan-huan HUANG Ya-nan LU Zai-qing ZHOU Xiang-dong ZHANG Haibin LI Qi LIU He-lu 

机构地区：[1]Department of Respiratory Medicine,The First Affiliated Hospital of Hainan Medical College,Haikou 570102,China [2]Hainan Respiratory Disease Clinical Medical Center,Haikou 571199,China [3]Institute of Deep-sea Science and Engineering,Chinese Academy of Sciences,Sanya 572000,China [4]Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266000,China

出　　处：《Journal of Hainan Medical University》2023年第23期1-6,共6页海南医学院学报（英文版）

基　　金：National Key R&D Plan(2022YFC2305004);Hainan Province Major Science and Technology Special Project(No.ZDKJ2021036);Key R&D projects in Hainan Province(No.ZDYF2020223);Hainan Province Key R&D Plan International Science and Technology Cooperation Project(GHYF2022011);Hainan Provincial Innovation Team Project(No.820CXTD448);National Natural Science Foundation of China(No.82260001,82160012)。

摘　　要：Objective:Tribulus terrestris saponin is a traditional Chinese medicine in China.This experiment was designed to investigate the effects of tribulus terrestris saponin on the proliferation and invasion ability of non-small cell lung cancer A549 cells.Methods:A549 cells were divided into normal control and experimental groups(Tribulus terrestris saponin 250μg/mL group,Tribulus terrestris saponin 200μg/mL group,Tribulus terrestris saponin 150μg/mL group,Tribulus terrestris saponin 100μg/mL group,Tribulus terrestris saponin 50μg/mL group).The proliferation viability of the cells in each group was detected by CCK8,the invasion of tumor cells was detected by Transwell model.The mRNA expression of MMP9 and caspase-3 in each group of cells was detected by RT-PCR.Immunofluorescence staining was used to observe the fluorescence intensity of caspase-3 in each group of cells.Results:Compared with the normal control group,tribulus terrestris saponin significantly inhibited the proliferation activity and invasion ability of A549 cells,which was statistically significant(P<0.01).In the invasion assay,compared with the control group,MMP9 expression was significantly reduced and caspase-3 expression was significantly increased in the tribulus terrestris saponin group,and both were concentration-dependent,with statistically significant differences(P<0.01).By cellular immunofluorescence staining experiments,it was found that the fluorescence expression of caspase-3 was enhanced in the experimental group compared with the normal control group,in which the high concentration saponin group was significantly higher than the low concentration group.Conclusion:Tribulus terrestris saponin can inhibit the invasive ability of A549 cells by down-regulating the expression of MMP9,and induce irreversible apoptosis by up-regulating the activation of caspase-3 expression to form caspase-3.

关 键 词：Tribulus terrestris saponin Non-small cell carcinoma Cysteinyl aspartate specific proteinase Matrix metallo-proteinase 

分 类 号：R734.2[医药卫生—肿瘤]