基于网络药理学探讨四物汤治疗宫颈癌的作用机制  

Mechanism of Siwu Decoction in the Treatment of Cervical Cancer Based on Network Pharmacology

作　　者：李锦湘 余田甜 李伟 丁玲玲 Li Jinxiang;Yu Tiantian;Li Wei;Ding Lingling(The First Clinical College,Hubei University of Traditional Chinese Medicine,Wuhan 430000,China)

机构地区：[1]湖北中医药大学第一临床学院,湖北武汉430000

出　　处：《巴楚医学》2024年第1期55-61,共7页Bachu Medical Journal

基　　金：湖北省中医药管理局2023—2024年度中医药青年人才项目(No:ZY2023Q004)。

摘　　要：目的:基于网络药理学探讨四物汤治疗宫颈癌的作用机制。方法:通过中药系统药理学数据库与分析平台知识库收集中药活性成分,利用治疗靶点数据库、GeneCards数据库收集疾病靶点,绘制韦恩图,利用Cytoscape3.10.1构建“药物-活性成分-疾病靶点”关系网络图以及绘制蛋白质互相关系网,将交集靶点基因进行基因本体(GO)富集分析及京都基因和基因组百科全书(KEGG)通路富集分析,并应用分子对接验证药物成分与靶点之间的作用。结果:四物汤治疗宫颈癌的有效成分有106个,关键靶标51个,关键靶点包括核受体共激活因子2(PNRC2)、前列腺素内过氧化物合酶2(PTGS2)等。通过蛋白质-蛋白质相互作用(PPI)分析得到四物汤治疗宫颈癌的核心靶点,包括苏氨酸激酶1(AKT1)、肿瘤坏死因子(TNF)、PTGS2、原癌基因转录因子(JUN)及胱天蛋白酶3(CASP3)等。分子对接分析证明豆甾醇与TNF、AKT1靶点具有良好的结合活性。结论:四物汤可通过调节TNF、AKT1关键靶点发挥治疗宫颈癌的作用,为临床应用提供了一定依据。Objective:To study the mechanism of action of Siwu decoction in the treatment of cervical cancer based on network pharmacology.Methods:The active ingredients were collected through the traditional Chinese medicine systems pharmacology knowledge base,and the disease targets were collected by therapeutic target database and GeneCards databases.Then the Venn diagram was drawn.The"drug-active ingredient-disease target"relationship network diagram was constructed by Cytoscape 3.10.1 and the protein interrelationship network was drawn.The intersection target genes were analyzed by gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis.The molecular docking was used for verification.Results:There were 106 active ingredients and 51 key targets in the treatment of cervical cancer,including nuclear receptor coactivator 2(PNRC2)and prostaglandin peroxide synthase 2(PTGS2).The core targets of Siwu decoction in the treatment of cervical cancer were obtained by protein-protein interaction(PPI)analysis,including threonine kinase 1(AKT1),tumor necrosis factor(TNF),PTGS2,proto-oncogene proteins c-jun(JUN),caspase-3(CASP3),etc.Molecular docking analysis showed that stigmasterol had good binding activity with TNF and AKT1 targets.Conclusion:Siwu decoction can play a role in the treatment of cervical cancer by regulating key targets TNF and AKT1,which provides a certain basis for clinical application.

关 键 词：四物汤 宫颈癌 网络药理学 肿瘤坏死因子 苏氨酸激酶1 

分 类 号：R737.33[医药卫生—肿瘤]