兔源肠致病性大肠埃希菌全基因组测序及毒力和耐药基因分析  

Whole Genome Sequencing Reveals the Distribution of Resistance and Virulence Genes of Enteropathogenic Escherichia Coli from Rabbits

作　　者：董浩 赵明海[1] 刘志国 李楠 邢壮壮 张乐颖 左琴[1] 董青花 梁春南[1] 刘佐民[1] 马丽颖[1] DONG Hao;ZHAO Minghai;LIU Zhiguo;LI Nan;XING Zhuangzhuang;ZHANG Leying;ZUO Qin;DONG Qinghua;LIANG Chunnan;LIU Zuomin;MA Liying(National Institutes for Food and Drug Control,National Rodent Laboratory Animal Resources Center,Beijing 102629,China)

机构地区：[1]中国食品药品检定研究院国家啮齿类实验动物资源库,北京102629

出　　处：《实验动物科学》2024年第1期70-76,共7页Laboratory Animal Science

摘　　要：目的揭示一株从腹泻实验兔中分离到的肠致病性大肠埃希菌ILAREc-01的基因组特征、毒力基因和耐药基因分布情况。方法采用高通量测序法对基因组序列进行测定,采用VFDB、ARDB、CAZy、SWISS-PROT、COG、CARD、KEGG、T3SS等8个数据库对基因组中的基因功能进行预测,并且对ILAREc-01菌株进行了遗传进化分析。结果ILAREc-01基因组中编码5249个基因;CARD数据库分析表明ILAREc-01菌株具有7类耐药机制的59个耐药基因;通过VFDB数据库分析,在ILAREc-01中注释了553个毒力基因,并发现了肠细胞脱落位点毒力岛(LEE);ILAREc-01与FORC028、E2865和110512株具有较高的同源性。结论本研究进行了兔源肠致病性大肠埃希菌ILAREc-01的全基因组测序,完成了毒力基因和耐药基因的注释,并开展了遗传进化分析,为揭示该病原的致病机制和科学防控实验兔大肠埃希菌病提供了数据支撑。Objective The aim of this study was to investigate the genomic characteristics,virulence genes and antibiotic resistance genes of enteropathogenic Escherichia coli ILAREC-01 isolated from experimental rabbits with diarrhea.Method High-throughput sequencing was performed to determine the whole genome sequence of ILAREC-01.Eight databases including VFDB,ARDB,CAZy,SwisS-Prot,COG,CARD,KEGG and T3SS were used to annotate the function of the predict genes.In addition,the phylogenetic features of ILAREC-01 strain was also described.Result The genome of ILAREC-01 contains 5249 genes.The analysis of the genome through CARD database showed that ILAREc-01 strain contains 59 resistance genes of 7 types of resistance mechanisms.Through VFDB database analysis,553 virulence genes were annotated in ILAREc-01.The Locus of enterocyte effacement pathogenicity island(LEE)was also identified in the genome of ILAREc-01.Phylogenetic trees analysis suggested that ILAREc-01 strain was highly homologous with FORC028、E2865 and 110512 strains.Conclusion In this study,the whole genome of rabbit enteropathogenic E.coli ILAREc-01 was sequenced,and the virulence genes,antibiotic resistance genes and evolution characteristics were also analyzed,which provided a reference for studying pathogenicity of enteropathogenic E.coli,and also provided data support for scientific prevention and control of colibacillosis in rabbits.

关 键 词：肠致病性大肠埃希菌 兔 全基因组测序 毒力基因 耐药基因 

分 类 号：Q95-3[生物学—动物学]