环状RNA检测方法研究进展  

Research progress of circRNA detection methods

作　　者：陈中强 袁发浒[1] 李莹[1] 施璐[1] 曹晓琴[1] 刘巍[2] 刘亮[2] CHEN Zhong-qiang;YUAN Fa-hu;LI Ying;SHI Lu;CAO Xiao-qin;LIU Wei;LIU Liang(School of Medicine,Jianghan University,Wuhan 430026,China;Department of Pharmacy,Zhongnan Hospital of Wuhan University,Wuhan 430071,China)

机构地区：[1]江汉大学医学院,武汉430026 [2]武汉大学中南医院,武汉430071

出　　处：《药物分析杂志》2024年第2期185-194,共10页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(No.82002252);中华国际医学交流基金会。

摘　　要：环状RNA(circRNA)是一大类内源性单链RNA,不同于其他线性RNA,circRNA通过外显子、内含子或2个外显子-内含子的反向剪接和融合形成共价闭环而产生,在高度分化的真核生物中普遍表达,并且与生物体的多种发育和代谢疾病过程密切相关。circRNA具有结构稳定、抗RNA酶降解、高度保守以及组织特异性表达等特点,是诊断和预后的理想生物标记物。传统方法如Northern印迹法、qRT-PCR和微阵列分析虽然提供了一定有用的信息,但是依旧受制于各自的缺点,无法在临床检验中大规模推广。近年来,为了解决这些问题,出现了一些新的检测方法。本文总结了目前circRNA检测方法的相关进展,阐述其优点和局限性,并讨论面临的挑战以及未来的发展方向。CircRNAs are a large class of endogenous single-stranded RNAs that are different from other linear RNAs,which are produced by back-splicing and fusion of either exons,introns,or both exon-intron into cova⁃lently closed loops.They are widely expressed in highly differentiated eukaryotes,and are closely related to vari⁃ous development and metabolic disease processes of organisms.They are characterized by stable structure,resist⁃ant to RNA degradation,conservation,and tissue-specific expression,making them ideal biomarkers for diagno⁃sis and prognosis.Traditional methods such as Northern blotting,qRT-PCR and microarray analysis provide use⁃ful information,however,they are subject to their own shortcomings.Traditional methods are restricted in large-scale promotion in clinical trials.In recent years,in order to solve these problems,some new detection methods have emerged.In this article,we reviewed the relevant progress of all current circRNA detection methods,expounded their advantages and limitations,and discussed the challenges and future development directions.

关 键 词：环状RNA检测 新方法 生物标志物 扩增 优点和缺点 

分 类 号：R917[医药卫生—药物分析学]