基于酶解法和化学计量学的黄精多糖差异性分析  

Polygonati Rhizoma polysaccharide differential analysis based on enzymolysis and chemometrics

作　　者：刁卓 胡冲 杨青山[3] 张亚中 DIAO Zhuo;HU Chong;YANG Qing-shan;ZHANG Ya-zhong(Anhui Medical College,Hefei 230031,China;Anhui Provincial Institute for Food and Drug Control,Key Laboratory of Quality Research and Evaluation of Traditional Chinese Medicines of the State Food and Drug Administration,Hefei 230051,China;Anhui University of Chinese Medicine,Hefei 230012,China)

机构地区：[1]安徽医学高等专科学校,合肥230031 [2]安徽省食品药品检验研究院国家药监局中药质量研究与评价重点实验室,合肥230051 [3]安徽中医药大学,合肥230012

出　　处：《药物分析杂志》2024年第2期324-332,共9页Chinese Journal of Pharmaceutical Analysis

基　　金：安徽省药品监督管理局监管科学研究重点项目-常用中药材DNA分子鉴定研究。

摘　　要：目的:建立糖苷酶水解下不同品种黄精多糖酶解产物的HPLC指纹图谱,探讨不同品种黄精多糖的差异,为黄精药材多糖质量评价提供参考。方法:黄精多糖经果糖苷酶水解后,采用HPLC建立黄精的指纹图谱;并结合相似度(SA)分析、聚类分析(HCA)和主成分分析(PCA),对指纹图谱进行分析,研究不同品种黄精多糖的差异。结果:不同品种黄精的多糖HPLC指纹图谱存在差异,共得到17个特异性寡糖片段,均含有果糖、葡萄糖和蔗糖。分析表明,3种基原黄精的种内指纹色谱相似度较高,差异较小,种间差异较大,而黄精混伪品与基原黄精差异明显。结论:不同品种黄精及其混伪品多糖酶解产物的指纹图谱结合化学计量学分析可以成功将黄精按照品种进行分类。所建立的HPLC方法简便、准确,重复性好,可用于黄精药材的多糖的差异研究。Objective:To determine the polysaccharide hydrolysates from Polygonati Rhizoma under glycoside enzymatic hydrolysis’HPLC fingerprint.Examine the variations among the polysaccharides produced by the various varieties of Polygonati Rhizoma and provide references for the assessment of the polysaccharide quality.Methods:After the polysaccharide from Polygonati Rhizoma was hydrolyzed by fructose enzymes,its fingerprint was established by HPLC-ELSD.The fingerprint was then analyzed using similarity analysis(SA),hierarchical cluster analysis(HCA),and principal component analysis(PCA)to determine the differences between the poly⁃saccharides from various Polygonati Rhizoma varieties.Results:Polysaccharides from various strains of Polygonati Rhizoma had different HPLC-ELSD fingerprints,and a total of 17 distinct oligosaccharide fragments were discov⁃ered,all of which contained fructose,glucose,and sucrose.Analysis revealed that there are significant intra species differences,minor differences,and high levels of similarity between the three types of Polygonati Rhizoma.The real Polygonati Rhizoma differs significantly from the imitation in several important ways.Conclusion:Polygo⁃nati Rhizoma can be successfully classified according to varieties,using the fingerprint of polysaccharide hydroly⁃sates of the various varieties of Polygonati Rhizoma and its adulterants.The developed HPLC method can be used for the differential analysis of polysaccharides in Polygonati Rhizoma and is straightforward,precise,and repeatable.

关 键 词：黄精 多糖 酶解法 指纹图谱 差异 化学计量学 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]