一种抗稻曲病菌的摩氏假单胞菌JP2-207及其抗性机制初探  

A Preliminary Study on the Mechanism of Pseudomonas mosselii JP2-207 Against Rice False Smut Fungus Ustilaginoidea virens

作　　者：刘思慧 明珂 陈国庆 冯国忠 LIU Sihui;MING Ke;CHEN Guoqing;FENG Guozhong(China National Rice Research Institute,Hangzhou 310006,China;Taizhou Agricultural Ecological Protection and Quality Safety Center,Taizhou,Zhejiang 318000,China)

机构地区：[1]中国水稻研究所,杭州310006 [2]台州市农业生态保护与质量安全中心,浙江台州318000

出　　处：《中国稻米》2024年第2期13-17,共5页China Rice

基　　金：浙江省重点研发计划项目(2021C02006);中央级公益性科研院所基本科研业务费(CPSIBRF-CNRRI-202115)。

摘　　要：从水稻根际表面土壤分离到一株能有效抑制稻曲病菌(Ustilaginoidea virens)生长的细菌菌株JP2-207。16s r RNA基因片段扩增及系统发育树分析表明,JP2-207属于摩氏假单胞菌(Pseudomonas mosselii)。测序分析显示,JP2-207全基因组为1个大小5 702 443 bp的环状DNA,包含5 154个蛋白编码基因,22个r RNA和78个t RNA基因。生物信息学分析预测了9个与次生代谢产物生物合成相关的基因簇,其中多个基因簇产生的代谢物被报道对植物病原菌具有抑制活性。本研究为摩氏假单胞菌JP2-207及其代谢产物防治水稻稻曲病的研究奠定了初步的理论基础。Pseudomonas mosselii JP2-207 was isolated from the soil of the rhizosphere of rice plants,this strain showed obvious inhibitory activity against Ustilaginoidea virens,an important rice pathogen.The complete genome of P.mosselii JP2-207 consists of 5702443 bp with a circle chromosome,5154 protein-coding genes,22 rRNA genes,and 78 tRNA.The genomic analysis revealed that 9 candidate gene clusters were involved in the biosynthesis of secondary metabolites.Together,this study provides preliminary results for exploring the antagonistic mechanism of rice false smut by P.mosselii JP2-207 and its secondary metabolites.

关 键 词：摩氏假单胞菌 水稻 稻曲病 次生代谢产物 生物合成基因簇 生物防治 

分 类 号：S511[农业科学—作物学] S435.111.4