反硝化硫氧化工艺中功能菌株的筛选、识别及验证  

SCREENING,IDENTIFICATION,AND VALIDATION OF FUNCTIONAL BACTERIAL STRAINS IN DENITRIFICATION AND DESULFURIZATION PROCESS

作　　者：徐冉 崔建东[1] 郜爽 黄聪 XU Ran;CUI Jiandong;GAO Shuang;HUANG Cong(School of Biological Engineering,Tianjin University of Science and Technology,Tianjin 300457,China;Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]中国科学院天津工业生物技术研究所,天津300308

出　　处：《环境工程》2023年第12期123-130,共8页Environmental Engineering

基　　金：天津市合成生物技术创新能力提升行动“环境与能源安全合成微生物组”(TSBICIP-CXRC-007)。

摘　　要：反硝化硫氧化工艺是处理含硫含氮有机废水生物处理技术中最具潜力的污水处理技术之一。为了解反硝化硫氧化工艺中发挥主要功能的菌属信息,同步运行3个膨胀颗粒污泥床反应器,测定反硝化硫氧化效能,解析活性污泥微生物群落结构变化,分离筛选出活性污泥中的菌株并进行功能验证。结果表明:3个反应器稳定运行后均可实现100 mg/L NO_(3)^(-)-N和100 mg/L Ac^(-)-C的100%去除,最高可以去除90%的200 mg/L S^(2-);16S rRNA分析表明:稳定运行反应器活性污泥的微生物群落结构和多样性中,相对丰度较高的5个菌属为Azoarcus、Pseudomonas、Thauera、Arthrobacter和Desulfomicrobium;采用平板分菌和流式分选2种分菌方式共得到分属于19个菌属的50株菌,综合活性污泥群落结构和碳氮硫污染物的去除率,初步确定Azoarcus、Thauera、Pseudomonas、Acinetobacter和Agrobacterium为反硝化硫氧化工艺的功能菌属。The denitrifying sulfur oxidation process is one of the most potential sewage treatment technologies in biological treatment for treating sulfur-containing and nitrogen-containing organic wastewater.In order to understand the information of bacteria that mainly play a role in the denitrifying sulfur oxidation process,three expanded granular sludge bed reactors were operated simultaneously to determine the oxidation efficiency of sulfur denitrification,analyze the changes of the microbial community structure of activated sludge,isolate and screen out the strains in activated sludge and verify their function.The results showed that 100%of 100 mg/L NO_(3)^(-)-N and 100 mg/L Ac^(-)-C can be removed after stable operation of the three reactors,and up to 90%of 200 mg/L S^(2-)can be removed.16S rRNA analysis was used to analyze the microbial community structure and diversity of activated sludge in the stable operation reactor,and found that the five genera with high relative abundance were Azoarcus,Pseudomonas,Thauera,Arthrobacter and Desulfomicrobium.A total of 50 strains belonging to 19 genera were obtained by plate separation and flow cytometry separation,and the removal rate of carbon,nitrogen and sulfur pollutants was synthesized to preliminarily determine that Azoarcus,Thauera,Pseudomonas,Acinetobacter and Agrobacterium were the functional genera of denitrified sulfur oxidation process.

关 键 词：反硝化硫氧化工艺 膨胀颗粒污泥床反应器 颗粒活性污泥 功能菌分离筛选 微生物群落结构 

分 类 号：X172[环境科学与工程—环境科学] X703