miRNA-21、白细胞介素-17、缺氧诱导因子-1α在多发性骨髓瘤患者中的表达水平及临床意义  

Expression levels and clinical significance of miRNA-21,interleukin-17 and hypoxia-inducible factor-1αin patients with multiple myeloma

作　　者：杨晓煜[1] 朱登勤 庞素蕾[1] 王盼盼 马若巾[1] 马云云 宋春鸽[1] YANG Xiaoyu;ZHU Dengqin;PANG Sulei;WANG Panpan;MARuojin;MAYunyun;SONG Chunge(Department of Hematology,the Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,He’nan,China)

机构地区：[1]郑州大学第五附属医院血液内科,郑州450052

出　　处：《癌症进展》2024年第2期214-218,共5页Oncology Progress

摘　　要：目的探讨微小RNA-21(miRNA-21)、白细胞介素-17(IL-17)、缺氧诱导因子-1α(HIF-1α)在多发性骨髓瘤患者中的表达及临床意义。方法选取78例多发性骨髓瘤患者和64例健康体检者,分别作为研究组和对照组。采用实时荧光定量聚合酶链反应(qPCR)检测两组受试者miRNA-21水平,酶联免疫吸附试验检测两组受试者血清IL-17、HIF-1α水平。比较两组受试者、不同预后情况多发性骨髓瘤患者miRNA-21、IL-17、HIF-1α水平。采用Pearson相关分析法分析多发性骨髓瘤患者miRNA-21与IL-17、HIF-1α水平的相关性。采用Cox风险比例回归模型分析多发性骨髓瘤患者预后的影响因素。采用受试者工作特征(ROC)曲线分析miRNA-21、IL-17、HIF-1α单独及联合检测对多发性骨髓瘤的诊断价值及对预后的预测价值。比较不同miRNA-21、IL-17、HIF-1α表达情况多发性骨髓瘤患者的生存情况。结果研究组患者miRNA-21、IL-17、HIF-1α水平均明显高于对照组,差异均有统计学意义(P﹤0.01)。预后不良多发性骨髓瘤患者miRNA-21、IL-17、HIF-1α水平均明显高于预后良好患者,差异均有统计学意义(P﹤0.01)。多发性骨髓瘤患者miRNA-21与IL-17、HIF-1α水平均呈正相关(r=0.447、0.462,P﹤0.05)。miRNA-21、IL-17、HIF-1α均是多发性骨髓瘤患者预后的独立危险因素(P﹤0.05)。ROC曲线显示,miRNA-21、IL-17、HIF-1α联合检测诊断多发性骨髓瘤的曲线下面积(AUC)、灵敏度、特异度、阳性预测值、阴性预测值均高于三者单独检测;miRNA-21、IL-17、HIF-1α联合检测预测多发性骨髓瘤患者预后的AUC、灵敏度、阴性预测值均高于三者单独检测。miRNA-21、IL-17、HIF-1α高表达组多发性骨髓瘤患者的2年累积生存率分别低于miRNA-21、IL-17、HIF-1α低表达组患者,差异均有统计学意义(P﹤0.05)。结论多发性骨髓瘤患者中miRNA-21、IL-17、HIF-1α均高表达,且miRNA-21与IL-17、HIF-1α水平均呈正相关,三�Objective To investigate the expression levels and clinical significance of microRNA-21(miRNA-21),interleukin-17(IL-17)and hypoxia-inducible factor-1α(HIF-1α)in patients with multiple myeloma.Method A total of 78 multiple myeloma patients and 64 healthy subjects were selected as study group and control group,respectively.Realtime fluores cence quantitative polymerase chain reaction(qPCR)was used to detect miRNA-21 level,and enzyme linked immunosorbent assay was used to detect serum IL-17 and HIF-1αlevels.The levels of miRNA-21,IL-17 and HIF-1αin two groups and multiple myeloma patients with different prognosis were compared.The correlation between miRNA-21 level and IL-17,HIF-1αlevels in multiple myeloma patients was analyzed by Pearson correlation analysis.Cox proportional hazard regression model was used to analyze the prognostic factors of patients with multiple myeloma.The diagnostic value and prognostic value of miRNA-21,IL-17 and HIF-1αsingle and combined detection in multiple myeloma were analyzed by receiver operating characteristic(ROC)curve.The survival of multiple myeloma patients with different expressions of miRNA-21,IL-17 and HIF-1αwere compared.Result The levels of miRNA-21,IL-17 and HIF-1αin study group were significantly higher than those in control group,and the differences were statistically significant(P<0.01).The levels of miRNA-21,IL-17 and HIF-1αin multiple myeloma patients with poor prognosis were significantly higher than those in patients with good prognosis,and the differences were statistically significant(P<0.01).The level of miRNA-21 was positively correlated with IL-17 and HIF-1αlevels in multiple myeloma patients(r=0.447,0.462,P<0.05).miRNA-21,IL-17 and HIF-1αwere independent prognostic risk factors in multiple myeloma patients(P<0.05).ROC curve showed that the area under the curve(AUC),sensitivity,specificity,positive predictive value and negative predictive value of miRNA-21,IL-17 and HIF-1αcombined detection in the diagnosis of multiple myeloma were higher than those

关 键 词：多发性骨髓瘤 MIRNA-21 白细胞介素-17 缺氧诱导因子-1Α 相关性 预后 诊断价值 

分 类 号：R733.3[医药卫生—肿瘤]