西沙马尾藻共附生真菌Aspergillus sp.SYPUF41304次级代谢产物的分离与鉴定  

Isolation and identification of secondary metabolites of epiphytic fungus Aspergillus sp.SYPUF41304 from Sargassum sp.in the Xisha islands

作　　者：齐鑫 陈伟豪 林秀萍[2] 肖姣 王俊锋[2] 刘永宏 QI Xin;CHEN Weihao;LIN Xiuping;XIAO Jiao;WANG Junfeng;LIU Yonghong(Wuya College of Innovation,Shenyang Pharmaceutical University,Shenyang 110016,China;CAS Key Laboratory of Tropical Marine Bio-resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangdong Key Laboratory of Marine Materia Medica,Guangzhou 510301,China)

机构地区：[1]沈阳药科大学,无涯创新学院,辽宁沈阳110016 [2]中国科学院南海海洋研究所,中国科学院热带海洋生物资源与生态重点实验室,广东省海洋药物重点实验室,广东广州510301

出　　处：《沈阳药科大学学报》2024年第2期182-187,共6页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金项目(82104061);广东省海洋药物重点实验室开放课题(LMM2022-1);辽宁省兴辽英才项目(XLYC1802037)。

摘　　要：目的对西沙马尾藻共附生真菌Aspergillus sp.SYPUF41304的次级代谢产物进行分离与鉴定。方法利用硅胶柱色谱、凝胶柱色谱、中压反相柱色谱及半制备高效液相色谱等分离手段对马尾藻共附生真菌Aspergillus sp.SYPUF41304的液体发酵提取物进行分离纯化,并利用核磁共振、质谱等波谱学方法,结合文献对照,确定化合物结构。结果最终从提取物中分离得到10个化合物,经鉴定为吲哚-3-甲醛(1H-indole-3-carboxaldehyde)(1)、吲哚-3-乙酸甲酯(methyl indole-3-acetate)(2)、环(L-脯氨酸-L-缬氨酸)(cyclo-L-prolyl-L-valine)(3)、brevianamide F(4)、asperterreusine A(5)、talaisocoumarin A(6)、3-甲基-6-羟基-8甲氧基-3,4-二氢异香豆素(3-methyl-6-hydroxy-8-methoxy-3,4-dihydroisocoumarin)(7)、3-甲氧基-6,8-二羟基-3-甲基-3,4-二氢异香豆素(3-methoxy-6,8-dihydroxy-3-methyl-3,4-dihydroisocoumarin)(8)、4-hydroxykigelin(9)和de-O-methyldiaporthin(10)。结论化合物按结构类型可分为生物碱和聚酮类等,化合物4~8均为首次从该属真菌中分离得到。所有化合物在4 mg·mL^(-1)浓度下,均未显示出抗细菌活性。Objective To isolate and identifiy the secondary metabolites of the epiphytic fungus Aspergillus sp.SYPUF41304 from Sargassum sp.in the Xisha islands.Methods Silica gel column,Sephadex LH-20,medium pressure reversed phase column chromatography and semi-preparative high performance liquid chromatography(HPLC)were used to separate and purify the extract from liquid fermentation of the strain Aspergillus sp.SYPUF41304.Results Ten compounds were isolated from the extract and identified as 1H-indole-3-carboxaldehyde(1),methyl indole-3-acetate(2),cyclo-(L-prolyl-L-valine)(3),brevianamide F(4),asperterreusine A(5),talaisocoumarin A(6),3-methyl-6-hydroxy-8-methoxy-3,4-dihydroisocoumarin(7),3-methoxy-6,8-dihydroxy-3-methyl-3,4-dihydroisocoumarin(8),4-hydroxykigelin(9),and de-O-methyldiaporthin(10).Conclusion The compounds could be divided into alkaloids and polyketones based on their structural type.Compounds 4-8 were isolated from the genus Aspergillus sp.for the first time.All the compounds showed no antibacterial activity at the concentration of 4 mg·mL^(-1).

关 键 词：马尾藻 共附生真菌 Aspergillus sp. 次级代谢产物 结构鉴定 

分 类 号：R914[医药卫生—药物化学]