microRNA-497在胰腺癌患者血清中定量检测及其临床意义  

Quantitative detection of microRNA-497in serum of patients with pancreatic cancer and its clinical significance

作　　者：程冰 刘少平[2,3] 高艳 张海[5] 胡亚华 卫银芝[2] 聂磊[6] CHENG Bing;LIU Shao-ping;GAO Yan;ZHANG Hai;HU Ya-hua;WEI Yin-zhi;NIE Lei(Wuhan University of Science and Technology,430081Wuhan,Hubei,China;Department of General Practice of Huangshi Central Hospital of Edong Healthcare Group,435000Huangshi,Hubei,China;Hubei Province Key Laboratory of Occupational Hazard Identification and Control,Wuhan University of Science and Technology,430062Wuhan,Hubei,China;The First Clinical Medical College of Hubei Polytchnic University,435000 Huangshi,Hubei,China;Department of Gastroenterology of Huangshi Central Hospital of Edong Healthcare Group,435000 Huangshi,Hubei,China;Department of Abdominal Tumor Surgery of Hubei Province Cancer Hospital,430069,Wuhan,Hubei,China)

机构地区：[1]武汉科技大学,湖北武汉430081 [2]鄂东医疗集团黄石市中心医院全科医学科,湖北黄石435000 [3]武汉科技大学职业危害识别与控制湖北省重点实验室,湖北武汉430062 [4]湖北理工学院第一临床学院,湖北黄石435000 [5]鄂东医疗集团黄石市中心医院消化内科,湖北黄石435000 [6]湖北省肿瘤医院腹部肿瘤外科,湖北武汉430079

出　　处：《临床消化病杂志》2024年第1期11-15,共5页Chinese Journal of Clinical Gastroenterology

基　　金：湖北省卫生健康科研基金资助项目(No:WJ2019H184,WJ2021F027)。

摘　　要：[目的]定量检测胰腺癌(pancreatic cancer,PC)患者血清microRNA-497(miR-497)表达量,探讨其临床意义。[方法]检测70例PC患者(PC组)治疗前、30位健康者(正常对照组)血清miR-497表达量以及血清CA199、CEA含量;随访PC患者治疗后预后2年;分析血清miR-497表达量的临床价值。[结果]PC组治疗前血清miR-497表达量显著低于正常对照组(P<0.01);血清miR-497对PC的诊断敏感度显著高于血清CA199(91.0%∶76.6%,χ2=7.637,P<0.01);两者诊断准确度比较无明显差异(P>0.05),三者联合检测可提高诊断敏感性和特异性。治疗前血清miR-497表达量与PC浸润深度、淋巴结转移及是否远处转移呈负相关(均P<0.01)。治疗前血清miR-497低表达组其治疗后1年、2年生存率显著低于高表达组(P<0.05)。Cox多因素分析显示治疗前血清miR-497表达量是影响PC预后的独立高危因素。[结论]血清miR-497低表达与PC侵袭转移、不良预后密切相关,对其诊断有一定价值,可成为PC临床辅助诊断、预后评价的指标。[Objective]To quantitatively detect the expression of serum micro-RNA-497(miR-497)in patients with pancreatic cancer(PC)and to explore its clinical significance.[Methods]Real-time fluorescence quantitative RT-PCR was used to detect the expression of miR-497in serum of 70patients with PC before treatment and 30healthy controls,and the levels of CA199and CEA in serum were detected at the same time.Patients with PC were followed up for 2years after treatment.Clinical value of the expression of serum miR-497was analyzed.[Results]The expression of serum miR-497in PC group before treatment was significantly lower than that in normal control group(P<0.01).The sensitivity of serum miR-497in the diagnosis of pancreatic cancer was significantly higher than that of serum CA199(91.0%vs 76.6%,χ2=7.637,P<0.01).There was no significant difference in diagnostic accuracy between the two groups(P>0.05).The combined detection of the three items could improve the sensitivity and specificity of diagnosis.The expression of serum miR-497before treatment was negatively correlated with the depth of PC invasion,lymph node metastasis and distant metastasis(P<0.01).The 1-year and 2-year survival rate in the group with low expression of serum miR-497before treatment was significantly lower than that in the group with high expression after treatment(P<0.05).Cox multivariate analysis showed that the expression of serum miR-497before treatment was an independent high risk factor affecting the prognosis of PC.[Conclusion]The low expression of serum miR-497is closely related to the invasion,metastasis and poor prognosis of PC.It has a certain value in the diagnosis of PC,and can be used as an index for clinical auxiliary diagnosis and prognosis evaluation of PC.

关 键 词：胰腺癌 microRNA-497 实时荧光定量RT-PCR 侵袭转移 预后 

分 类 号：R735.9[医药卫生—肿瘤]