五种滇西中草药的总黄酮含量测定及抗菌、抗氧化活性初步研究  

Determination of Total Flavonoids and Preliminary Study on Antibacterial and Antioxidant Activities of Five Western Yunnan Chinese Herbs

作　　者：李自鸿 刘衡 陈佳洁 许艳飞 戴鑫 刘颖琳 LI Zi-hong;LIU Heng;CHEN Jia-jie;XU Yan-fei;DAI Xin;LIU Ying-lin(College of Pharmacy,Dali University,Yunnan Dali 671000;Yunnan Key Laboratory of Screening and Research on Anti-pathogenic Plant Resources from West Yunnan(Cultivation),Yunnan Dali 671000;Jianchuan Branch of Dali Tobacco Company,Yunnan Dali 671300,China)

机构地区：[1]大理大学药学院,云南大理671000 [2]云南省滇西抗病原植物资源筛选研究重点实验室(培育),云南大理671000 [3]大理州剑川县烟草公司,云南大理671300

出　　处：《广州化工》2023年第21期70-73,112,共5页GuangZhou Chemical Industry

基　　金：云南省滇西抗病原植物资源筛选研究重点实验室(培育)开放课题(APR202103);国家自然科学基金资助项目(8186074);云南省科技计划项目(202001AT070021);2022年云南省大学生创新创业训练计划项目(S202210679024)。

摘　　要：以五种滇西中草药为原料,对其醇提物进行总黄酮含量的测定及抗菌抗氧化活性的研究。结果表明鞭打绣球的总黄酮含量较高,含量为(363.47±1.35)μg/mg。五种中草药均表现出广谱抑菌活性,MIC值为3.13~25.00 g/mL,MBC值为3.13~25.00 g/mL。五种中草药表现出较强的自由基清除能力,其中掌叶梁王茶清除能力最强,清除DPPH自由基和ABTS自由基的IC50分别为61.61 mg/L和8.54 mg/L。本实验为滇西中草药在天然防腐剂中的开发利用提供了研究基础。The content of total flavonoids and the antibacterial and antioxidant activities of the ethanol extracts of five western Yunnan Chinese herbal medicines were determined.The results showed that the total flavone content of Hemiphragma heterophyllum was(363.47±1.35)μg/mg.The five Chinese herbal medicines all showed broad-spectrum antibacterial activity,with MIC value of 3.13~25.00 g/mL and MBC value of 3.13~25.00 g/mL.Five kinds of Chinese herbal medicine showed strong free radical scavenging capacity,among which the scavenging capacity of Nothopanax delavayi was the strongest,and IC50 of DPPH free radicals and ABTS free radicals were 61.61 mg/L and 8.54 mg/L,respectively.A research basis was provided for the development of western Yunnan herbal medicine in natural preservatives.

关 键 词：滇西药物 总黄酮含量 抗氧化活性 抗菌活性 

分 类 号：R284.2[医药卫生—中药学]