Extracellular vesicle-carried GTF2I from mesenchymal stem cells promotes the expression of tumor-suppressive FAT1 and inhibits stemness maintenance in thyroid carcinoma  

作　　者：Jie Shao Wenjuan Wang Baorui Tao Zihao Cai Haixia Li Jinhong Chen 

机构地区：[1]Department of General Surgery,Huashan Hospital,Fudan University,Shanghai 200040,China [2]Department of Pathology,Huashan Hospital,Fudan University,Shanghai 200040,China

出　　处：《Frontiers of Medicine》2023年第6期1186-1203,共18页医学前沿（英文版）

基　　金：supported by Shanghai Shenkang Hospital Development Center(No.SHDC12019X38).

摘　　要：Through bioinformatics predictions,we identified that GTF2I and FAT1 were downregulated in thyroid carcinoma(TC).Further,Pearson’s correlation coefficient revealed a positive correlation between GTF2I expression and FAT1 expression.Therefore,we selected them for this present study,where the effects of bone marrow mesenchymal stem cell-derived EVs(BMSDs-EVs)enriched with GTF2I were evaluated on the epithelial–to–mesenchymal transition(EMT)and stemness maintenance in TC.The under-expression of GTF2I and FAT1 was validated in TC cell lines.Ectopically expressed GTF2I and FAT1 were found to augment malignant phenotypes of TC cells,EMT,and stemness maintenance.Mechanistic studies revealed that GTF2I bound to the promoter region of FAT1 and consequently upregulated its expression.MSC-EVs could shuttle GTF2I into TPC-1 cells,where GTF2I inhibited TC malignant phenotypes,EMT,and stemness maintenance by increasing the expression of FAT1 and facilitating the FAT1-mediated CDK4/FOXM1 downregulation.In vivo experiments confirmed that silencing of GTF2I accelerated tumor growth in nude mice.Taken together,our work suggests that GTF2I transferred by MSC-EVs confer antioncogenic effects through the FAT1/CDK4/FOXM1 axis and may be used as a promising biomarker for TC treatment.

关 键 词：thyroid carcinoma mesenchymal stem cell extracellular vesicle GTF2I FAT1 CDK4 

分 类 号：R736.1[医药卫生—肿瘤]