基于条件优化探索对流免疫电泳技术的实验教学设计  

Optimizing Experimental Conditions for Convective Immunoelectrophoresis in Teaching Experiments

作　　者：康艳华[1] 姚佳露 杜斌豪 KANG Yan-hua;YAO Jia-lu;DU Bin-hao(Department of Immunology and Pathogenic Biology,School of Basic Medicine,Hangzhou Normal University,Hangzhou 310000,China)

机构地区：[1]杭州师范大学基础医学院,浙江杭州310000

出　　处：《教育教学论坛》2024年第5期121-124,共4页Education And Teaching Forum

基　　金：2022年度浙江省高校实验室工作研究项目“实验教学中对流免疫电泳实验条件的改进及优化”(YB202204);2022年度杭州师范大学基础医学院教改项目“对流免疫电泳实验条件的改进及优化”(JCYXYJG202209)。

摘　　要：为了提高对流免疫电泳实验的成功率和缩短电泳时间,本实验以BSA和自制备的兔抗BSA抗血清作为实验材料,通过优化和改变电泳介质、电泳缓冲液等条件,观察比较抗原-抗体沉淀线形成的位置、形状情况来探索符合实验教学要求的过程方案。结果表明,在100 V电压下,采用TAE作为缓冲液、1.0%~1.2%的琼脂凝胶作为电泳介质时,电泳15~20分钟内可形成白色、清晰可见的抗原-抗体沉淀线,实验效果最佳。In order to improve the success rate of the convective immunoelectrophoresis experiment and shorten the time of electrophoresis,BSA and self-made rabbit anti-BSA anti-serum were used as experimental materials in this experiment.By optimizing and changing the conditions of electrophoresis media and electrophoresis buffer,the position and shape of the antigen-antibody precipitation line were observed and compared to explore the process scheme that met the requirements of experimental teaching.The results showed that when TAE was used as buffer and 1.0%~1.2%agar gel was used as electrophoresis medium at 100 V,white and visible antigen-antibody precipitation lines could be formed within 15~20 minutes of electrophoresis,and the experimental effect was the best.

关 键 词：对流免疫电泳 实验教学 条件优化 

分 类 号：R3[医药卫生—基础医学]