樟芝-太子参双向液体发酵培养基优化及效应分析  

Formulation of Antrodia camphorata Culture Medium Incorporated with Pseudostellaria heterophylla By-product

作　　者：赵生月 吴小环 姜春玲 张余 郑世仲 刘盛荣 叶祖云 ZHAO Shengyue;WU Xiaohuan;JIANG Chunling;ZHANG Yu;ZHENG Shizhong;LIU Shengrong;YE Zuyun(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China;Research Center of Fujian Province Characteristic Medicinal Plants,Ningde,Fujian 352100,China;College of Life Science,Ningde Normal University,Ningde,Fujian 352100,China;College of Food Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]福建省特色药用植物工程技术研究中心,福建宁德352100 [3]宁德师范学院生命科学学院,福建宁德352100 [4]福建农林大学食品科学学院,福建福州350002

出　　处：《福建农业学报》2024年第1期75-82,共8页Fujian Journal of Agricultural Sciences

基　　金：福建省科技厅对外合作项目(2021I0046);福建省科技计划高校产学合作项目(2021N5005);宁德师范学院科研项目(2022ZX01)。

摘　　要：【目的】有效利用太子参根须副产物资源,提高樟芝(Antrodia camphorata)菌丝产量,促进活性成分合成。【方法】以太子参根须(粉末)作为培养基组分,Plackett-Burman试验筛选影响樟芝菌丝生长的关键成分,正交试验优化培养基组成。【结果】筛选试验结果表明,葡萄糖、酵母粉、蛋白胨及KH2PO4对樟芝菌丝生长有重要影响,正交试验优化的培养基组成为:太子参6 g·L^(−1),葡萄糖10 g·L^(−1),酵母粉4 g·L^(−1),蛋白胨6 g·L^(−1),KH_(2)PO_(4)1.5g·L^(−1)。利用优化培养基深层发酵,樟芝生物量达到4.73 g·L^(−1),与对照培养基及PDB培养基相比,胞内三萜含量分别提高2.75%和24.85%,胞外多糖分别提高161.11%和113.64%;发酵液中高密度无性孢子含量达1.8×10^(7)个·mL^(-1)。【结论】太子参适合作为樟芝双向液体发酵的药性成分,可有效促进樟芝菌丝生长、活性成分合成和无性孢子形成,是太子参根须副产物资源利用新途径。【Objective】To utilize a Pseudostellaria heterophylla by-product for increasing Antrodia camphorata yield and bio-effectiveness,formulation of a culture medium was optimized.【Methods】Powder of P.heterophylla fine roots was included in formulating a medium to maximize the mycelial growth of A.camphorata by a Plackett-Burman orthogonal experimental design.【Results】In addition to 6 g·L^(−1) of P.heterophylla powder,the medium was optimized to contain the ingredients critical to the mushroom growth,which included glucose 10 g·L^(−1),yeast extract 4 g·L^(−1),peptone 6 g·L^(−1),and KH_(2)PO_(4)1.5 g·L^(−1).The A.camphorata culture on the formulated medium produced 4.73 g·L^(−1) biomass,which yielded 2.75%more intracellular triterpenoids than control and 24.85%more than PDB medium,and 161.11%more exopolysaccharides than control and 113.64%more than PDB medium.The fermentation broth also contained a high asexual spore at a density of 1.8×10^(7) spores·mL^(−1).【Conclusion】P.heterophylla root powder could plausibly be added to the medium for A.camphorate culture as it promoted the mycelial growth,bioactive constituents yield,and asexual spore production of the mushroom.

关 键 词：樟芝 太子参 正交试验 培养基优化 活性成分 

分 类 号：Q93[生物学—微生物学]