微小甲状腺乳头状癌组织中Ⅲ类β-微管蛋白表达与临床病理特征的关系  

Relationship between class IIIβ-tubulin expression and clinicopathologic features in papillary thyroid microcarcinoma tissues

作　　者：彭丽红[1] 谢冰 吴波 刘亚群[2] 王正花[3] 王健[4] 王康 Peng Lihong;Xie Bing;Wu Bo;Liu Yaqun;Wang Zhenghua;Wang Jian;Wang Kang(Department of Pathology,Nanjing Tongren Hospital Affiliated to the School of Medicine,Southeast University,Nanjing,Jiangsu 211102,China;Department of Pathology,Nanjing DIAN Medical Laboratory Center Co.,Ltd.,Nanjing,Jiangsu 211103,China;Department of Otorhinolaryngology Head and Neck Surgery,Nanjing Tongren Hospital Affiliated to the School of Medicine,Southeast University,Nanjing,Jiangsu 211102,China;Department of Pediatrics,Nanjing Tongren Hospital Affiliated to the School of Medicine,Southeast University,Nanjing,Jiangsu 211102,China;Department of Anesthesiology,Nanjing Tongren Hospital Affiliated to the School of Medicine,Southeast University,Nanjing,Jiangsu 211102,China;Department of Pathology,Nanjing Hospital of Traditional Chinese Medicine,Nanjing,Jiangsu 210000,China)

机构地区：[1]东南大学医学院附属南京同仁医院病理科,江苏南京211102 [2]东南大学医学院附属南京同仁医院耳鼻咽喉头颈外科,江苏南京211102 [3]东南大学医学院附属南京同仁医院儿科,江苏南京211102 [4]东南大学医学院附属南京同仁医院麻醉科,江苏南京211102 [5]南京迪安医学检验中心有限公司病理科,江苏南京211103 [6]南京市中医院病理科,江苏南京210000

出　　处：《齐齐哈尔医学院学报》2024年第2期107-111,共5页Journal of Qiqihar Medical University

基　　金：南京市卫生科技发展专项资金项目(YKK22261)。

摘　　要：目的探究微小甲状腺乳头状癌组织中Ⅲ类β-微管蛋白(TUBB3)表达与临床病理特征的关系。方法收集2016年8月—2018年12月本院手术治疗留取的100例微小甲状腺乳头状癌组织及100例结节性甲状腺肿组织标本,采用免疫组化法检测TUBB3、E-钙黏蛋白(ECAD)和波形蛋白(VIMENTIN)表达情况,分析TUBB3与ECAD、VIMETIN表达及甲状腺微小乳头状癌(PTMC)临床病理特征的关系。结果微小甲状腺乳头状癌组织中TUBB3、VIMENTIN阳性表达率明显高于结节性甲状腺肿组织,ECAD阳性表达率明显低于结节性甲状腺肿组织(P<0.05);Pearson相关性分析显示,微小甲状腺乳头状癌组织中TUBB3表达与ECAD呈负相关,与VIMENTIN呈正相关(P<0.001);存在淋巴结转移、神经侵犯、脉管侵犯、被膜侵犯及肿瘤出芽率高患者TUBB3表达阳性率高于无淋巴结转移、神经侵犯、脉管侵犯、被膜侵犯及肿瘤出芽率低者(P<0.05);经术后3年随访,TUBB3阳性表达患者无疾病生存率低于TUBB3阴性表达者(P<0.05);多因素Cox比例风险回归分析结果显示,存在被膜侵犯、高肿瘤出芽率和TUBB3阳性表达是PTMC患者预后的独立危险因素(P<0.05)。结论TUBB3在微小甲状腺乳头状癌组织中表达升高,可能作用于上皮-间质转化过程促进肿瘤转移复发,影响预后。Objective To explore the relationship between expression of class IIIβ-tubulin(TUBB3)in papillary thyroid microcarcinoma(PTMC)tissues and clinicopathologic features.Methods 100 PTMC tissue samples and 100 nodular goiter tissue samples from surgical treatment were collected.The expressions of TUBB3,E-cadherin(ECAD)and vimentin(VIMENTIN)were detected by immunohistochemistry.The relationship between TUBB3 and ECAD,VIMETIN,and clinicopathological characteristics of PTMC was analyzed.Results The positive expression rates of TUBB3 and VIMENTIN in PTMC tissues were significantly higher than those in nodular goiter tissues,while positive expression rate of ECAD was significantly lower than that in nodular goiter tissues(P<0.05).Pearson correlation analysis showed that the expression of TUBB3 in PTMC tissues was negatively correlated with ECAD,while positively correlated with VIMENTIN(P<0.001).The positive expression rate of TUBB3 in patients with lymph node metastasis,nerve invasion,vascular invasion,capsule invasion and high tumor budding rate was higher than those in patients without lymph node metastasis,nerve invasion,vascular invasion and capsule invasion,and with low tumor budding rate(P<0.05).After 3-year of postoperative follow-up,disease-free survival rate in patients with positive TUBB3 was lower than that with negative TUBB3(P<0.05).The results of multivariate Cox proportional hazards regression analysis showed that capsule invasion,high tumor budding rate and TUBB3 positive expression were independent risk factors of prognosis of PTMC patients(P<0.05).Conclusions The expression of TUBB3 is up-regulated in PTMC tissues,which may act on the process of epithelial-mesenchymal transition to promote tumor metastasis and recurrence,and affects prognosis.

关 键 词：甲状腺微小乳头状癌 βⅢ-微管蛋白 临床病理特征 预后 

分 类 号：R736.1[医药卫生—肿瘤]