草麻黄根中一个新软木脂类化合物  

A new suberin from roots of Ephedra sinica Stapf

作　　者：张博文 李孟[1,2] 王小兰 杨颖[1,2] 周诗琪 陶思琦 杨梦 朱登辉 徐雅桐[1,2] 冯卫生 郑晓珂 ZHANG Bo-wen;LI Meng;WANG Xiao-lan;YANG Ying;ZHOU Shi-qi;TAO Si-qi;YANG Meng;ZHU Deng-hui;XU Ya-tong;FENG Wei-sheng;ZHENG Xiao-ke(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;The Engineering and Technology Center for Chinese Medicine Development of Henan Province,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]河南省中药开发工程技术研究中心,河南郑州450046

出　　处：《药学学报》2024年第3期661-666,共6页Acta Pharmaceutica Sinica

基　　金：国家重点研发计划-中医药现代化研究(2019YFC1708802);中央引导地方科技发展专项资金([2016]149);河南省高层次人才特殊支持计划(ZYQR201810080).

摘　　要：采用硅胶柱色谱、薄层色谱和半制备液相等多种色谱学技术从草麻黄根中分离得到6个化合物。根据理化性质与波谱数据鉴定其结构,分别为(Z)-阿魏酸二十二酯(1)、(E)-阿魏酸二十二酯(2)、邻苯二甲酸-双(2′-乙基庚基)酯(3)、2,2′-氧代双(1,4-二叔丁苯)(4)、邻苯二甲酸二异丁酯(5)、邻苯二甲酸二(2-乙基己基)酯(6)。其中化合物1为新化合物,化合物2~4是首次从麻黄植物中分离得到。采用皮质酮诱导大鼠肾上腺嗜铬细胞瘤(PC-12细胞)细胞损伤模型进行化合物活性筛选,结果表明,化合物1和5能显著改善皮质酮诱导的PC-12细胞损伤,并且显著升高细胞中5-HT7(5-hydroxytryptamine 7)受体蛋白的表达,具有潜在的抗抑郁活性。Six compounds were isolated from the roots of Ephedra sinica Stapf using various chromatographic techniques such as silica gel column chromatography,thin layer chromatography and semi-preparative HPLC.Their chemical structures were identified by analysis of physicochemical properties and spectral data,and determined as(Z)-docosanylferulate(1),(E)-docosanylferulate(2),bis(2-ethylheptyl)phythalate(3),2,2′-oxybis(1,4-di-tertbutylbenzene)(4),diisobutyl phthalate(5),bis(2-ethylhexyl)phthalate(6).Among them,compound 1 is a new compound,compounds 2−4 were first isolated from Ephedra.A corticosterone-induced PC-12 cell injury model was used for compound activity screening.The results showed that compounds 1 and 5 significantly improved corticosterone-induced PC-12 cell injury and significantly increased 5-HT7 receptor protein expression in the cells,indicating potential antidepressant activity.

关 键 词：麻黄根 化学成分 软木脂类 (Z)-阿魏酸二十二酯 抗抑郁活性 

分 类 号：R284[医药卫生—中药学]