微生物复合载体制备及包埋Halomonas sp.NH2B的硝化性能  

Preparation of Microbial Composite Carrier and Nitration Performance of Halomonas sp.NH2B Encapsulation

作　　者：苏鑫 王诗涵 刘佩武 周鹏 刘鹰[1,4] 吴英海 韩蕊 SU Xin;WANG Shihan;LIU Peiwu;ZHOU Peng;LIU Ying;WU Yinghai;HAN Rui(Key Laboratory of Environment Controlled Aquaculture of Ministry of Education,Dalian Ocean University,Dalian 116023,China;College of Marine Science and Environment,Dalian Ocean University,Dalian 116023,China;College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China;College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 310058,China;College of Ocean and Cicil Engineering,Dalian Ocean University,Dalian 116023,China)

机构地区：[1]大连海洋大学设施渔业教育部重点实验室,辽宁大连116023 [2]大连海洋大学海洋科技与环境学院,辽宁大连116023 [3]大连海洋大学水产与生命学院,辽宁大连116023 [4]浙江大学生物系统工程与食品科学学院,浙江杭州310058 [5]大连海洋大学海洋与土木工程学院,辽宁大连116023

出　　处：《环境科学与技术》2023年第11期1-10,共10页Environmental Science & Technology

基　　金：国家自然科学基金(32273186);辽宁省科学技术计划项目(2021JH2/10200012);大连市高层次人才创新支持计划“兴辽英才计划”资助(2021RQ089)。

摘　　要：包埋固定化技术可为微生物提供附着载体,提高循环水养殖系统曝气生物滤器(BAF)中微生物去除含氮污染物的能力。文章在聚乙烯醇-海藻酸钠(PVA-SA)复合载体材料基础上,添加0%~2%(w/w)的环糊精(β-CD)、硅藻土(DIA)和沸石(Z-13X),通过正交实验、理化性质、水质监测和扫描电子显微镜分析,考察复合载体机械强度、物化稳定性、可重复利用性、硝化性能和内部结构。结果表明:复合载体最佳复合比例为PVA 10%、SA 2%、β-CD 0%、DIA 2%、Z-13X 1%,该比例的复合载体机械性能良好,改性后的复合载体可适用于酸性尾水中,其内部呈现多孔结构,孔径适合微生物生长。同时,筛选出一株高效硝化菌株Halomonas sp.NH2B,将其包埋于复合载体中进一步研究,该微生物复合载体36 h可100%去除初始浓度为50 mg/L的NH_(4)^(+)-N,且7个循环周期后,复合载体孔隙结构基本保持不变,且有微生物附着,NH_(4)^(+)-N去除率仍可达76.93%。新型复合载体材料可有效解决微生物接种后生存能力弱和菌株流失的问题,保证硝化效率的同时提高可重复利用率。The embedding immobilization technology can provide a carrier for microorganisms to improve the ability of mi⁃croorganisms to remove nitrogen-containing pollutants in the biological aerated filter(BAF)of the recirculating aquaculture system.Based on polyvinyl alcohol-sodium alginate(PVA-SA)composite carrier material,0%~2%(w/w)cyclodextrin(β-CD),diatomite(DIA)and zeolite(Z-13X)were added.The mechanical strength,physical and chemical stability,reusability,nitrifi⁃cation performance and internal structure of the composite carrier were investigated by orthogonal experiment,physical and chemical properties,water quality monitoring and scanning electron microscope analysis.The results showed that the optimum composite ratio of composite carrier was PVA 10%,SA 2%,β-CD 0%,DIA 2%and Z 1%.The composite carrier with this ra⁃tio had good mechanical properties.The modified composite carrier could be applied to acidic tail water,and its internal struc ture was porous,and the pore size was suitable for microbial growth.At the same time,an efficient nitrifying strain Halomo⁃nas sp.NH2B was screened and embedded in a composite carrier for further research.The microbial composite carrier could remove 100% NH_(4)^(+)-N at an initial concentration of 50 mg/L after 36 hours,and after seven cycles,the pore structure of the composite carrier remained basically unchanged,and there were microorganisms attached.The NH_(4)^(+)-N removal rate could still reach 76.93%.In summary,the new composite carrier material can effectively solve the problems of weak viability and strain loss after microbial inoculation,ensure the nitrification efficiency and improve the reusability.

关 键 词：包埋 微生物固定化 生物载体 硝化菌 

分 类 号：X172[环境科学与工程—环境科学]