1株B基因型牛副流感3型病毒的分离鉴定及全基因组序列分析  

Isolation,identification and genome sequencing of BPIV3 genotype B

作　　者：张倩 王旭 韩猛立[1] 张星星[1] 吴桐忠[1] 钟发钢 黄新[1] 张乾义[2] ZHANG Qian;WANG Xu;HAN Mengli;ZHANG Xingxing;WU Tongzhong;ZHONG Fagang;HUANG Xin;ZHANG Qianyi(State Key Laboratory for Sheep Genetic Improvement and Healthy Production,Xinjiang Academy of Agricultural and Reclamation Science,Xinjiang 832000,China;China Institute of Veterinary Drug Control,Beijing 100081,China)

机构地区：[1]新疆农垦科学院省部共建绵羊遗传改良与健康养殖国家重点实验室,新疆石河子832000 [2]中国兽医药品监察所,北京100081

出　　处：《中国兽医学报》2024年第1期44-51,共8页Chinese Journal of Veterinary Science

基　　金：新疆农垦科学院优秀中青年人才培养引导计划专项资助项目(SKLSGIHP2021A02);新疆兵团重点领域科技攻关计划资助项目(2019AB029);中央级公益性科研院所基本科研业务费院级统筹资助项目(Y2022LM21)。

摘　　要：为探究新疆某集约化牛场1~6月龄犊牛群中牛副流感病毒3型(BPIV3)的感染情况,对采集的60份犊牛鼻拭子样品进行RT-PCR检测、病毒分离鉴定及全基因组测序和遗传进化分析。结果显示,样品中BPIV3核酸阳性率为11.67%。从核酸阳性样品中分离获得1株BPIV3,并命名为XJ21032-1(45),其基因组全长为15512 bp;遗传进化分析表明,XJ21032-1(45)属于BPIV3 B基因型,该分离株与澳大利亚的BPIV3 B基因型参考株Q5592(EU277658)的同源性最高为93.4%。本研究成功分离得到了1株B基因型BPIV3,证实B型毒株在我国的存在和流行,为BPIV3疫苗研发提供了原材料,也有助于我国BPIV3分子进化规律及溯源的进一步研究。To investigate the infection of bovine parainfluenza virus type 3 in herd of calves aged 1-6 months in an intensive cattle farm in Xinjiang,60 nasal swab samples were subjected to RT-PCR assay,virus isolation and identification,whole genome sequencing and genetic evolutionary analysis.The results showed that the nucleic acid positivity rate of BPIV3 was 11.67%.A strain of BPIV3 was isolated from the nucleic acid positive sample and named XJ21032-1(45),its genome length was 15512 bp.Genetic evolutionary analysis showed that XJ21032-1(45)belonged to BPIV3 genotype B,and the highest homology was 93.4%with the Australian BPIV3 genotype B reference strain Q5592(EU277658).This study successfully isolated a strain of B-genotype BPIV3,confirming the existence and prevalence of B-genotype strains in China,providing raw materials for the development of BPIV3 vaccines,and contributing to further research on the molecular evolution and traceability of BPIV3.

关 键 词：牛副流感病毒3型 B基因型 分离鉴定 全基因组序列分析 

分 类 号：S852.65[农业科学—基础兽医学]