菊茎叶总黄酮高效液相色谱指纹图谱与抗氧化活性的谱效关系研究  

Spectrum-Effect Relationship Between the HPLC Fingerprint of Total Flavonoids in Chrysanthemum Stems and Leaves and Antioxidant Activities

作　　者：陈慧芳 巫梦莹[1] 罗美美 程冉冉 丁杨飞 熊俊伟 张伟 吴德玲 CHEN Huifang;WU Mengying;LUO Meimei;CHENG Ranran;DING Yangfei;XIONG Junwei;ZHANG Wei;WU Deling(School of Pharmacy,Anhui University of Chinese Medicine,Anhui Hefei230012,China;Anqing Medical and Pharmaceutical College,Anhui Anqing246052,China;Traditional Chinese Medicine Processing and Inheritance Base,State Administration of Traditional Chinese Medicine,Anhui University of Chinese Medicine,Anhui Hefei230012,China;Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement,Anhui Hefei230012,China;Anhui Provincial Key Laboratory of New Manufacturing Technology for Traditional Chinese Medicine Decoction Pieces,Anhui Hefei230012,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]安庆医药高等专科学校,安徽安庆246052 [3]安徽中医药大学国家中医药管理局中药炮制传承基地,安徽合肥230012 [4]省部共建安徽道地中药材品质提升协同创新中心,安徽合肥230012 [5]中药饮片制造新技术安徽省重点实验室,安徽合肥230012

出　　处：《安徽中医药大学学报》2024年第2期96-102,共7页Journal of Anhui University of Chinese Medicine

基　　金：国家自然科学基金项目(81973485);安徽高校自然科学研究重点项目(KJ2020A0390,KJ2020A0889);安徽省高校优秀青年骨干教师国内访问研修项目(gxgnfx2022124)。

摘　　要：目的研究菊茎叶总黄酮指纹图谱与其抗氧化活性的相关性,探究菊茎叶总黄酮抗氧化活性的物质基础。方法采用高效液相色谱法建立11批菊茎叶总黄酮样品的指纹图谱;分别采用1,1-二苯基-2-三硝基苯肼自由基清除法、2,2′-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基清除法及血浆铁离子还原法测定其抗氧化活性,结合灰色关联度法、偏最小二乘回归法分析谱效关系。结果建立17个共有峰的菊茎叶总黄酮的高效液相色谱指纹图谱,指认其中11个共有峰,相似度大于0.886。灰色关联分析显示其中11个色谱峰关联度均大于0.9,偏最小二乘回归分析结果显示6个色谱峰的变量重要性投影值>1,表明菊茎叶总黄酮与抗氧化活性有重要相关性。结论共有峰6(山柰酚-3-O-芸香糖苷)、峰11、峰12(香叶木素-7-O-β-D-葡萄糖苷)、峰13(木犀草素)、峰15与抗氧化活性密切相关,初步推测为菊茎叶总黄酮抗氧化活性的物质基础。Objective To investigate the fingerprint of total flavonoids in chrysanthemum stems and leaves and its relationship with antioxidant activities,and to explore the material basis of the antioxidant activities of total flavonoids in chrysanthemum stems and leaves.Methods High-performance liquid chromatography(HPLC)was used to establish the fingerprints of 11 batches of total flavonoids from chrysanthemum stems and leaves,and 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging method,2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonate)(ABTS)radical scavenging method,and ferric reducing ability of plasma(FRAP)method were used to measure their antioxidant activities;spectrum-effect relationship was analyzed using grey correlation degree and partial least squares regression.Results The HPLC fingerprints of total flavonoids in chrysanthemum stems and leaves were established with 17 common peaks,among which 11 common peaks were identified with a similarity of greater than 0.886.The grey correlation analysis showed a correlation degree of>0.9 for all 11 chromatographic peaks,and the partial least squares regression analysis showed a variable importance projection value of>1 for 6 chromatographic peaks,suggesting a significant correlation with antioxidant activities.Conclusion The common peak 6(kaempferol-3-O-rutinoside),peak 11,peak 12(diosmetin-7-O-β-D-glucopyranoside),peak 13(luteolin),and peak 15 are closely associated with antioxidant activities and are speculated to be the material basis of antioxidant activities of total flavonoids from chrysanthemum stems and leaves.

关 键 词：菊茎叶 指纹图谱 抗氧化活性 谱效关系 

分 类 号：R284.1[医药卫生—中药学]