开放性实验:聚乙烯亚胺修饰碳点的合成、纯化与表征  

Opening experiments:synthesis,purification,and characterization of polyethyleneimine-modified carbon dots

作　　者：李瑞[1] 许贯虹[1] 于海琳[1] 岑瑶 彭艳[1] 沈凡丽 魏芳弟[1] LI Rui;XU Guanhong;YU Hailin;CEN Yao;PENG Yan;SHEN Fanli;WEI Fangdi(Nanjing Medical University,Nanjing 211166,China;Jiangsu Institute for Food and Drug Control,Nanjing 210019,China)

机构地区：[1]南京医科大学,江苏南京211166 [2]江苏省食品药品监督检验研究院,江苏南京210019

出　　处：《色谱》2024年第4期393-398,共6页Chinese Journal of Chromatography

基　　金：南京医科大学教育研究课题(2023ZD006);南京医科大学研究生优质教育资源建设项目(2023E004).

摘　　要：开放性实验是培养拔尖创新人才的有效手段。我们基于学生兴趣、科研热点和实验室条件,设计了实验方案。实验采用一步水热法,以柠檬酸(CA)为碳源,聚乙烯亚胺(PEI)为修饰剂,合成PEI修饰碳点(PEI-CDs)。首先,将CA、PEI在0.1 mol/L的盐酸中超声至完全溶解,混匀后转移到高压反应釜中,放入烘箱130℃反应2 h,制得PEI-CDs原液;然后将冷却至室温的原液旋转蒸发浓缩至2 mL,并用无水乙醇沉淀、洗涤;最后,将所得沉淀放入真空干燥箱,70℃干燥12 h得到PEI-CDs粉末。利用紫外-可见分光光度计、荧光分光光度计、红外光谱仪和透射电镜对所合成的PEI-CDs进行表征。结果表明,所采用的无水乙醇沉淀纯化方法简单、快速、经济、绿色,制得的PEI-CDs水溶性好,发光性能优异,大小均一,稳定性高。通过该开放性实验,学生不仅熟悉了大型仪器的使用,而且增强了科学研究的兴趣。Open experiments are an effective means of cultivating top-notch innovative talents.Based on student interests,research hotspots and our laboratory conditions,an experimental scheme was designed.In this experiment,polyethyleneimine modified carbon dots(PEI-CDs)were prepared via a one-step hydrothermal method using citric acid(CA)as the carbon source and PEI as the surface passivator.First,CA and PEI were completely dissolved in 0.1 mol/L HCl and transferred into an autoclave.The autoclave was heated to 130℃foR^(2)h.PEI-CDs solution was obtained.After cooling to room temperature,the solution was concentrated to 2 mL by rotary evaporation.Finally,the PEI-CDs were precipitated,washed with ethanol,and dried under vacuum at 70℃for 12 h.The obtained PEI-CDs were characterized by fluorescence spectrophotometry,absorption spectrophotometry,infrared spectrometry,and transmission electron microscopy.The results indicated that anhydrous-ethanol precipitation is a simple,rapid,economical,and green purification method.The as-prepared PEI-CDs had unique properties,such as good water solubility,high luminescence,uniform particle sizes,and good stability.Through this open experiment,students can not only master the operation of large-scale instruments but also enhance their interest in scientific research.

关 键 词：开放性实验 聚乙烯亚胺 碳点 荧光 纯化方法 

分 类 号：O658[理学—分析化学]