原子荧光光谱法检测食用鱼中汞、砷含量  

Atomic Fluorescence Spectroscopy for Detecting Mercury and Arsenic Content in Edible Fish

作　　者：吴志华 张斯益 WU Zhihua;ZHANG Siyi(Rugao Center for Disease Control and Prevention,Physical and Chemical Laboratory,Rugao 226500,China)

机构地区：[1]如皋市疾病预防控制中心理化检验科,江苏如皋226500

出　　处：《食品安全导刊》2024年第6期47-49,53,共4页China Food Safety Magazine

摘　　要：目的:建立原子荧光光谱法同时测定食用鱼中汞、砷含量的方法。方法:采用微波消解法对样品进行预处理,原子荧光光谱法同时测定食用鱼(淡水鱼、海水鱼)中汞、砷含量,分析其检测结果。结果:汞在0~10.0μg·mL^(-1)时线性关系良好,砷在0~40.0μg·mL^(-1)时线性关系良好,相关系数> 0.999。汞的检出限在2.50~3.83μg·mL^(-1),回收率为94.1%~99.0%,精密度为2.9%;砷的检出限在2.30~3.68μg·mL^(-1),回收率为93.3%~99.2%,精密度为2.0%。对比发现食用海水鱼中汞、砷含量显著高于淡水鱼(P <0.05)。结论:原子荧光光谱法操作简单、检测灵敏度较高,可同时测定食用鱼汞、砷含量。Objective:To establish a method for simultaneous determination of mercury and arsenic content in edible fish using atomic fluorescence spectroscopy.Method:Microwave digestion method was used to preprocess the sample,and atomic fluorescence spectroscopy was used to simultaneously determine the mercury and arsenic content in edible fish(freshwater and seawater fish),and the detection results were analyzed.Result:Mercury has a good linear relationship at 0~10.0μg·mL^(-1),while arsenic has a good linear relationship at 0~40.0μg·mL^(-1),with a correlation coefficient greater than 0.999.The detection limit of mercury is between 2.50μg·mL^(-1)and 3.83μg·mL^(-1),with a recovery rate of 94.1%to 99.0%and RSD of 2.9%;the detection limit of arsenic is between 2.30μg·mL^(-1) and 3.68μg·mL^(-1),the recovery rate is 93.3%~99.2%,the RSD is 2.0%.Comparison shows that the mercury and arsenic content in edible seawater fish is higher than that in freshwater fish(P<0.05).Conclusion:Atomic fluorescence spectroscopy is simple to operate and has high detection sensitivity,and can simultaneously determine the mercury and arsenic content in edible fish.

关 键 词：食用鱼 汞含量 砷含量 原子荧光光谱法 

分 类 号：O657.31[理学—分析化学] TS254.7[理学—化学]