施氏假单胞菌菌剂的好氧生长条件优化及其短程反硝化性能  

Optimization of aerobic growth conditions of a Stutzerimonas stutzeri agent and its partial denitrification performance

作　　者：蔡雨衡 程凯[1] CAI Yuheng;CHENG Kai(Hubei Key Laboratory of Ecological Restoration for River-Lakes and Algal Utilization,College of Resources and Environmental Engineering,Hubei University of Technology,Wuhan 430068,China)

机构地区：[1]湖北工业大学资源与环境工程学院,河湖生态修复与藻类利用湖北省重点实验室,武汉430068

出　　处：《华中师范大学学报（自然科学版）》2024年第2期184-196,共13页Journal of Central China Normal University：Natural Sciences

基　　金：湖北省大学生创新创业训练计划(202110500012)。

摘　　要：短程反硝化是目前生物脱氮技术的研究热点之一,但有关短程反硝化菌剂的研究仍较为匮乏.该文从污水厂活性污泥中分离了1株兼具异养氨氧化能力和短程反硝化能力的施氏假单胞菌(Stutzerimonas stutzeri WH-01).通过单因素优化试验,逐步确定了WH-01菌剂在好氧条件下的最适生长参数:以300 mg·L^(-1)氨氮为氮源、以1500 mg·L^(-1)葡萄糖为碳源、培养温度为35℃,初始pH为8.0.以乙酸钠为碳源时,WH-01菌剂虽具有全程反硝化能力,但会优先进行短程反硝化:在培养基中的亚硝氮积累效率为81%,在实际污水中的亚硝氮积累效率为97%.上述结果表明WH-01菌剂具有工程应用的潜力.Although partial denitrification is one of the hotspots in the researchfield of biological nitrogen removal technology,the study on the microbial agent for partial denitrification is still scarce.In this paper,a strain of Stutzerimonas stutzeri(also known as Pseudomonas stutzeri)WH-01 with both heterotrophic ammonia-oxidation ability and partial denitrification ability was isolated from the activated sludge of a sewage treatment plant.Through the single factor optimization experiments,the optimization of aerobic growth conditions of the WH-01 agent under aerobic condition were determined as:300 mg·L^(-1)ammonia nitrogen as nitrogen source,1500 mg·L^(-1)glucose as carbon source,culture temperature of 35℃and initial pH of 8.0.When using sodium acetate as the carbon source,although WH-01 agent had the ability of full denitrification,it conducted partial denitrification more preferentially with the nitrite accumulation efficiency of 81%in culture medium and 97%in actual sewage.This study indicated that WH-01 bactericide had the potential of engineering application.

关 键 词：菌剂 短程反硝化 施氏假单胞菌 好氧发酵 亚硝酸盐积累率 

分 类 号：X172[环境科学与工程—环境科学] X703.1