胰岛素样生长因子结合蛋白3在脑梗死模型的表达及Smad信号通路的参与机制  

Expression of insulin-like growth factor binding protein 3in cerebral infarction rats and its involvement in Smad signaling pathway

作　　者：张翠 吴孟海 多云妍 Zhang Cui;Wu Menghai;Duo Yunyan(Department of Neurology,Jinan People's Hospital,Jinan 271199,Shandong Province,China)

机构地区：[1]济南市人民医院神经内科,271199

出　　处：《中华老年心脑血管病杂志》2024年第3期331-335,共5页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：济南市卫生健康委科技计划项目(2021-2-16)。

摘　　要：目的 探讨Smad信号通路机制在脑梗死模型大鼠中的作用以及脑组织胰岛素样生长因子结合蛋白3(IGFBP-3)的表达变化与神经功能的关系。方法 60只健康成年雄性SD大鼠随机分为模型组、假手术组及正常对照组,每组20只,模型组采用线栓法建立脑梗死模型,假手术组仅暴露颈内动脉后直接缝合皮肤,建模成功后1周评估各组大鼠的改良神经功能缺损(mNSS)评分。采用苏木精-伊红染色法检测脑组织病理学变化情况,采用Western blot法对大鼠脑组织中IGFBP-3、Smad2、Smad4蛋白水平进行检测,并采用逆转录聚合酶链反应测定大鼠脑组织中IGFBP-3、Smad2、Smad4 mRNA水平。用Spearman相关性分析IGFBP-3、Smad2、Smad4、P21表达水平之间的相关性。结果 苏木精-伊红染色结果显示,模型组大鼠出现明显脑组织水肿,表现为脑细胞排列紊乱,小胶质细胞数量变多,脑细胞核仁出现模糊,其中脑梗死面积为20.55%。与假手术组和正常对照组比较,模型组大鼠mNSS评分、IGFBP-3、Smad2、Smad4蛋白及mRNA相对表达水平明显升高,P21蛋白及mRNA相对表达水平明显降低(P<0.05,P<0.01)。Spearman相关性分析显示,模型组大鼠脑组织中IGFBP-3 mRNA表达水平与mNSS评分、Smad2 mRNA、Smad4 mRNA呈显著正相关(r=0.568,r=0.623,r=0.597,P<0.01);IGFBP-3 mRNA表达水平与P21呈显著负相关(r=-0.573,P<0.01)。结论 脑梗死大鼠脑组织IGFBP-3水平显著升高,且IGFBP-3水平与脑梗死大鼠神经功能之间关系密切,其作用机制可能与Smad信号通路有关。Objective To investigate the role of Smad signaling pathway in rat model of cerebral in-farction and explore the expression of insulin-like growth factor binding protein 3(IGFBP-3)in brain tissue and its relationship with neural function.Methods Sixty healthy adult male SD rats were randomly and equally divided into model group,sham-operation group,and normal control group.The model of cerebral infarction was established by using intraluminal thread occlusion,and the rats of the sham-operation group were only given exposure of the internal carotid artery and direct suture of the incision.In 1week after successful modeling,Modified Neurological Seve-rity Score(mNSS)was used to evaluate the neurological function.HE staining was employed to observe the histopathological changes in the brain tissues.Western blotting and RT-PCR were adopted to detect the brain expression of IGFBP-3,Smad2and Smad4at protein and mRNA le-vels.Spearman correlation analysis was conducted to analyze the correlation among the expression levels of IGFBP-3,Smad2,Smad4and P21.Results HE staining displayed that obvious brain ede-ma,characterized by disordered arrangement of brain cells,increased microglia,and blurred nucleo-lus of brain cells were observed in the rats of the model group,with the area of cerebral infarct of 20.55%.The mNSS score and the protein and mRNA levels of IGFBP-3,Smad2and Smad4were significantly higher,but the P21protein and mRNA levels were obviously reduced in the model group than the sham-operation group and normal control group(P<0.05,P<0.01).Spearman correlation analysis showed that the mRNA level of IGFBP-3in cerebral infarction rats was posi-tively correlated with the mNSS score and mRNA expression levels of Smad2and Smad4(r=0.568,r=0.623,r=0.597;P<0.01),and negatively with P21 mRNA level in the brain tissue(r=-0.573;P<0.01).Conclusion The level of IGFBP-3is significantly increased in brain tissue of rats with cerebral infarction,and it is closely associated with neural function of these rats,which may be related t

关 键 词：胰岛素样生长因子结合蛋白质3 模型 动物 脑梗死 SMAD蛋白质类 数据相关性 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]