食管腺鳞癌组织p63和人表皮生长因子受体2蛋白表达与临床病理特征的关系及其对患者生存的影响  

Relationship of expressions of p63 and human epidermal growth factor receptor 2 proteins with clinicopathological features of esophageal adenosquamous carcinoma and their impact on patients'survival

作　　者：王美玲 陈曦 宋昕[2] 赵学科[2] 韩文莉 黄金 钟侃 胡景峰 丁倩倩 蒋宁宁 杨会杰 王立东[2] 岳文彬 Wang Meiling;Chen Xi;Song Xin;Zhao Xueke;Han Wenli;Huang Jin;Zhong Kan;Hu Jingfeng;Ding Qianqian;Jiang Ningning;Yang Huijie;Wang Lidong;Yue Wenbin(Department of Oncology,Puyang Oilfi eld General Hospital Affi liated to Xinxiang Medical University,Xinxiang 457001,China;State Key Laboratory of Esophageal Cancer Prevention and Treatment and Henan Key Laboratory for Esophageal Cancer Research of the First Affi liated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]新乡医学院附属濮阳油田总医院肿瘤科,河南新乡457001 [2]郑州大学第一附属医院省部共建食管癌防治国家重点实验室,河南郑州450052

出　　处：《实用肿瘤杂志》2024年第2期155-160,共6页Journal of Practical Oncology

基　　金：国家自然科学基金(81872032);河南省卫生系统出国研修计划项目(2017097)。

摘　　要：目的分析p63和人表皮生长因子受体2(human epidermal growth factor receptor 2,HER2)蛋白表达在食管腺鳞癌(esophageal adenosquamous carcinoma,EASC)患者中的临床意义和预后价值。方法回顾性分析来自郑州大学第一附属医院省部共建食管癌防治国家重点实验室已建立的80万例食管癌临床诊疗和随访信息数据库和样本库的2005年12月至2021年12月确诊的115例EASC患者的临床资料。采用亲和素-生物素-过氧化物酶复合物(avidin-biotin-peroxidase complex,ABC)技术检测p63和HER2蛋白的表达。采用χ^(2)检验分析p63和HER2蛋白表达与患者临床病理特征间的关系。应用KaplanMeier法分析蛋白表达与患者预后的关系。采用Cox回归分析评估患者预后的影响因素。结果EASC患者男女比例为3.1∶1。男性和女性EASC患者在各临床病理特征方面比较,差异均无统计学意义(均P>0.05)。p63阳性患者的肿瘤家族史和淋巴结转移率均低于p63阴性患者(均P<0.05)。HER2阳性患者男性占比和淋巴结转移率均高于HER2阴性患者(均P<0.05)。p63和HER2阳性患者总生存均优于阴性患者(均P<0.05)。多因素Cox回归分析显示,p63表达、性别、分化程度和TNM分期均是EASC患者预后的独立影响因素(均P<0.05)。结论p63和HER2蛋白表达与EASC患者的预后及淋巴结转移密切相关,有望成为EASC患者的预后标志物及潜在治疗靶点。Objective To analyze the clinical significance and prognostic value of the expressions of p63 and human epidermal growth factor receptor 2(HER2)proteins in patients with esophageal adenosquamous carcinoma(EASC).Methods The clinical data of 115 EASC patients diagnosed from December 2005 to December 2021 were retrospectively collected from 800000 cases in the esophageal cancer clinical diagnosis,treatment and follow-up information database and sample database established by State Key Laboratory of Esophageal Cancer Prevention and Treatment and Henan Key Laboratory for Esophageal Cancer Research of the First Affiliated Hospital of Zhengzhou University.Avidin-biotin-peroxidase complex(ABC)technique was used to detect the expressions of p63 and HER2 proteins.χ^(2) test was conducted to analyze the association between the protein expressions of p63 and HER2 and the clinicopathological characteristics of the patients.Kaplan Meier method was used to analyze the association between the expressions of the two proteins and the prognosis of patients.Multivariate Cox regression was used to evaluate the influencing factors of patients'prognosis.Results In this cohort,the male to female ratio of EASC patients was 3.1∶1,and there was no significant difference in clinicopathological characteristics between male and female EASC patients(all P>0.05).The proportion of patients with family history of tumors and the rate of lymph node metastasis in the p63 pos-itive group were both significantly lower than those in the p63 negative group(both P<0.05).The proportion of male patients and the rate of lymph node metastasis in the HER2 positive group were both significantly higher than those in the HER2 negative group(both P<0.05).The p63 and HER2 positive groups had significantly better survival than the corresponding negative groups(both P<0.05).Multivariate Cox regression analysis found that p63 expression,gender,degree of differentiation,and TNM stage were all independent influencing factors of the prognosis of EASC patients(all P<0

关 键 词：食管腺鳞癌 P63 人表皮生长因子受体2 生存分析 

分 类 号：R735.1[医药卫生—肿瘤]