Hyperion系统分析直肠癌肿瘤微环境与新辅助放化疗疗效及预后的相关性  

Correlation between tumor microenvironment and efficacy and prognosis of neoadjuvant chemoradiotherapy for rectal cancer:A Hyperion imaging system analysis

作　　者：陈虹 张继红[2] 赵建国[2] 乌新林[3] 郝钦[3] 郭佳星 邢国圣 安志玲 宝莹娜[2] CHEN Hong;ZHANG Jihong;ZHAO Jianguo;WU Xinlin;HAO Qin;GUO Jiaxing;XING Guosheng;AN Zhiling;BAO Yingna(Affiliated Hospital of Inner Mongolia Medical University,Inner Mongolia Hohhot 010050,China;Department of Radiotherapy,Affiliated Hospital of Inner Mongolia Medical University,Inner Mongolia Hohhot 010050,China;Department of Gastrointestinal Surgery,Affiliated Hospital of Inner Mongolia Medical University,Inner Mongolia Hohhot 010050,China)

机构地区：[1]内蒙古医科大学附属医院,内蒙古呼和浩特010050 [2]内蒙古医科大学附属医院放射治疗科 [3]内蒙古医科大学附属医院胃肠外科,内蒙古呼和浩特010050

出　　处：《现代肿瘤医学》2024年第8期1452-1457,共6页Journal of Modern Oncology

基　　金：内蒙古科技计划项目(编号:2019GG150);内蒙古自然科学基金项目(编号:2019MS08124)。

摘　　要：目的:探索局部晚期直肠癌(locally advanced rectal cancer,LARC)患者肿瘤微环境特征与新辅助放化疗(neoadjuvant chemoradiotherapy,NCRT)疗效及预后的相关性。方法:回顾性分析2020年10月至2021年4月于我院行NCRT的4例LARC患者,将26个金属标签抗体对患者石蜡切片进行标记,然后用Hyperion成像系统检测和分析标记样本,并与临床疗效和预后进行分析。结果:4例LARC患者NCRT后缓解程度及预后存在显著差异,在成像系统中观察到3种不同的肿瘤微环境特征,包括免疫细胞富集型、免疫细胞匮乏型和免疫抑制细胞为主型,CD8^(+)T细胞、Foxp3^(+)调节性T细胞、CD56^(+)自然杀伤细胞、CD14^(+)单核细胞、CD11b^(+)巨噬细胞、CD11c^(+)树突状细胞和增殖细胞抗原(Ki-67)均在各自样本中显著高表达(P<0.05),PD-1和PD-L1仅在患者2中高表达。结论:LARC患者具有明显的肿瘤微环境异质性,且其异质性可导致患者NCRT疗效及预后的差异。Objective:To explore the correlation between tumor microenvironment characteristics and the efficacy and prognosis of neoadjuvant chemoradiotherapy(NCRT)in locally advanced rectal cancer(LARC)patients.Methods:Four LARC patients who received NCRT in our hospital from October 2020 to April 2021 were retrospectively analyzed.26 metal-labeled antibodies were used to label paraffin sections of patients,and the labeled samples were then detected and analyzed with the Hyperion imaging system and the clinical efficacy and prognosis were analyzed.Results:There were significant differences in the degree of remission and prognosis after NCRT in four LARC patients.Three different tumor microenvironment characteristics were observed in Hyperion system,including immune cell enriched type,immune cell deficient type and immunosuppressive cell dominant type.CD8^(+)T cells,Foxp3^(+)regulatory T cells,CD56^(+)natural killer cells,CD14^(+)monocytes,CD11b^(+)macrophages,CD11c^(+)dendritic cells and Ki-67 were significantly expressed in their respective samples(P<0.05).PD-1 and PD-L1 were only highly expressed in patient two.Conclusion:LARC patients have significant tumor microenvironment heterogeneity,and its heterogeneity can lead to differences in the efficacy and prognosis of patients after NCRT.

关 键 词：局部晚期直肠癌 新辅助放化疗 肿瘤微环境 疗效 预后 

分 类 号：R735.3[医药卫生—肿瘤]