外周血神经丝蛋白轻链多肽含量与神经压迫性疼痛程度相关性研究  

Correlation between the content of peripheral blood neurofilament light chain polypeptides and the extent of neural compressive pain

作　　者：李城 姚吉祥 金世光 伏小阳 LI Cheng;YAO Jixiang;JIN Shiguang;FU Xiaoyang(Department of Pain,the Affiliated Taizhou People′s Hospital of Nanjing Medical University,Nanjing Medical University,Taizhou,Jiangsu 225300,China;Department of Central Laboratory,the Affiliated Taizhou People′s Hospital of Nanjing Medical University)

机构地区：[1]南京医科大学附属泰州人民医院疼痛科,江苏泰州225300 [2]南京医科大学附属泰州人民医院中心实验室

出　　处：《徐州医科大学学报》2024年第2期119-123,共5页Journal of Xuzhou Medical University

基　　金：江苏省博士后资助项目(2019Z156);泰州市人民医院院级课题(ZL201929)。

摘　　要：目的探讨外周血神经丝蛋白轻链多肽(Nefl)水平与神经根性颈椎病(CSR)疼痛程度的相关性,并评估其是否可作为CSR进展的生物标志物。方法健康SD大鼠18只,随机数字表法分为2组,Sham组(9只)和大鼠坐骨神经慢性压迫损伤模型(CCI)组(9只)。Sham组暴露坐骨神经后缝合皮肤,CCI组暴露坐骨神经后先环扎再缝合皮肤;于术前,术后1、3、7、14 d采用电子测痛仪检测2组大鼠机械缩足反应阈值(MWT);并采集外周血进行定量蛋白质组分析,采用ELISA方法检测Nefl蛋白表达水平。将80例CSR患者作为研究组,同期体检的24例健康人群作为对照组,收集疼痛视觉模拟评分(VAS)结果,采用ELISA方法检测外周血Nefl蛋白表达;Spearman秩相关系数法评价外周血Nefl表达水平随疼痛程度的变化规律。结果CCI组大鼠术前,术后1、3、7、14 dMWT持续下降;3、7、14 d时MWT显著低于Sham组(P<0.01);TMT标记定量蛋白质组分析显示Nefl蛋白表达显著上调,Log(foldchange)=1.14,P<0.05;CCI组大鼠术前,术后1、3、7、14 d外周血Nefl蛋白表达量持续上升,Sham组3、7、14 d逐渐下降,MWT与大鼠外周血Nefl蛋白表达量呈负相关(r=-0.662,P<0.01)。CSR患者外周血Nefl表达显著高于健康人群(P<0.01),VAS为8分患者显著高于VAS为6分的患者(P<0.01),VAS与外周血Nefl呈正相关(r=0.41,P<0.01)。结论CCI模型大鼠和CSR患者外周血Nefl表达显著增加,且与疼痛程度相关,Nefl可作为临床上CSR病情进展评估的生物标志物。Objective To explore the correlation between the levels of peripheral blood neurofilament light chain peptide(Nefl)and the severity of pain in cervical spondylotic radiculopathy(CSR),and assess whether Nefl can serve as a biomarker for the progression of CSR.Methods According to the random number table method,18 healthy SD rats were divided into two groups(n=9):a Sham group and a chronic constriction injury(CCI)group.In the Sham group,the sciatic nerve was exposed and the skin was sutured.In the CCI group,the sciatic nerve was exposed and the skin was sutured after being looped.Then,their mechanical withdrawal thresholds(MWT)were measured by an electronic pain meter before operation and on post-operation days 1,3,7,and 14.Peripheral blood samples were collected for quantitative proteomic analysis.The levels of peripheral blood Nefl were detected by ELISA.Furthermore,80 patients with CSR were selected as an experimental group,and 24 healthy individuals who underwent physical examination at the same time were selected as acontrol group.The visual analog scale(VAS)scores were collected,and the levels of Nefl protein in peripheral blood was detected by ELISA.The Spearman rank correlation coefficient method was used to evaluate the correlation between the levels of Nefl in peripheral blood and the severity of pain.Results Rats in the CCI group showed consistent declines in MWT before operation and on post-operation days 1,3,7,and 14,and its MWT values on postoperation days 3,7,and 14 significantly lower than those in the Sham group(P<0.01).According to the TMT-marked quantitative proteomic analysis,Nefl resulted in significantly up-regulated protein expression,with Log(foldchange)=1.14 and P<0.05.Furthermore,the rats in the CCI group presented consistent increases in Nefl protein contents of the peripheral blood before operation and on post-operation days 1,3,7,and 14.In contrast,the Sham group showed gradual decreases in Nefl protein contents on post-operation days 3,7,and 14.There was a negative correlation between

关 键 词：神经根型颈椎病 神经丝蛋白轻链多肽 坐骨神经慢性压迫损伤模型 疼痛视觉模拟评分 生物标志物 定量蛋白质组分析 

分 类 号：R744.2[医药卫生—神经病学与精神病学]