电针对硝酸甘油致偏头痛小鼠疼痛行为及三叉神经血管系统的影响  

Effect of electro-acupuncture on pain behavior and trigeminovascular system in nitroglycerine induced migraine mice

作　　者：徐晓白[1] 张若滔 王雪飞[1] 温雅丽[1] 王握瑜 刘璐[1] 赵洛鹏 李志娟 王麟鹏[1] 郭静[1] 李彬[1] XU Xiaobai;ZHANG Ruotao;WANG Xuefei;WEN Yali;WANG Woyu;LIU Lu;ZHAO Luopeng;LI Zhijuan;WANG Linpeng;GUO Jing;LI Bin(Acupuncture Center of Beijing Hospital of Traditional Chinese Medicine,Capital Medical University/Beijing Key Laboratory of Acupuncture and Moxibustion Neuromodulation,Beijing 100010,China)

机构地区：[1]首都医科大学附属北京中医医院针灸中心/针灸神经调控北京市重点实验室,北京100010

出　　处：《现代中西医结合杂志》2024年第3期293-298,305,共7页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金资助项目(82004452);北京市科学技术学会青年人才托举工程(BYESS2023337);北京市医院管理局“扬帆”计划重点医学专业(重点扶持专业)-中西医结合康复(ZYLX202140)。

摘　　要：目的观察电针对硝酸甘油致偏头痛小鼠疼痛行为及三叉神经血管功能的影响,探讨其可能作用机制。方法将20只C57BL/6雄性小鼠随机分为对照组、模型组、假针组、电针组,每组5只。除对照组外,其余组小鼠于实验第1,3,5,7,9,11,13天给予10 mg/kg硝酸甘油腹腔注射建立慢性偏头痛模型。在每次注射后30 min,电针组和假针组进行电针干预。实验当天(第0天)注射前及实验第2,4,6,8,10,12,14天分别检测小鼠眶周机械痛阈及后足机械痛阈、热痛痛阈;实验第14天,采用ELISA法检测血清一氧化氮(NO)和降钙素基因相关肽(CGRP)水平,采用免疫荧光和Western blot法检测三叉神经节中c-Fos蛋白表达情况。结果实验第6,8,10,12,14天,模型组小鼠的眶周机械刺激反应评分均明显高于同期对照组(P均<0.05);实验第10,12,14天,电针组小鼠的眶周机械刺激反应评分均明显低于同期模型组(P均<0.05),且电针组小鼠实验第12,14天的眶周机械刺激反应评分均明显低于同期假针组(P均<0.05)。实验第8,10,12,14天,模型组小鼠的后足50%撤退阈值均明显低于同期对照组(P均<0.05),后足热痛撤退潜伏期均明显短于同期对照组(P均<0.05);实验第10,12,14天,电针组小鼠的后足50%撤退阈值均明显高于同期模型组(P均<0.05),后足热痛撤退潜伏期均明显长于同期模型组(P均<0.05),且电针组小鼠实验第12,14天的后足50%撤退阈值明显高于同期假针组(P均<0.05),后足热痛撤退潜伏期均明显长于同期假针组(P均<0.05)。模型组小鼠血清NO、CGRP水平和三叉神经节中c-Fos阳性表达平均光密度、c-Fos蛋白相对表达量均明显高于对照组(P均<0.05),而电针组各指标均明显低于模型组(P均<0.05)。结论电针能明显改善硝酸甘油致慢性偏头痛小鼠的疼痛行为,其可能是通过降低血清NO、CGRP水平及三叉神经敏化蛋白c-Fos的表达,从而改善三叉神经血管功能障碍实现的。Objective It is to observe the effect of electro-acupuncture(EA)on pain behavior and trigeminovascular system in nitroglycerine induced migraine mice,and to clarify its internal mechanism.Methods Twenty male C57BL/6 mice were randomly divided into control group,model group,sham acupuncture group and EA group,with 5 mice in each group.The mice of all groups except for the control group were given nitroglycerin by intraperitoneal injection on Day 1,3,5,7,9,11,13 of experiment to establish models of chronic migraine.The sham acupuncture group and EA group were respectively treated with sham EA and EA at 30 min after each injection.The periorbital mechanical pain threshold,hindfoot mechanical pain threshold and thermal pain threshold of the mice were respectively detected before injection on the day of the experiment(day 0)and on days 2,4,6,8,10,12,and 14 of the experiment;on day 14 of the experiment,the serum levels of nitric oxide(NO)and calcitonin gene-related peptide(CGRP)were detected by ELISA,and the expression of c-Fos protein in trigeminal ganglion was detected by immunofluorescence and Western blot.Results On days 6,8,10,12,and 14,the periorbital mechanical stimulation response scores of mice in the model group were significantly higher than those of mice in the control group during the same period(all P<0.05);on days 10,12,and 14,the periorbital mechanical stimulation response scores of mice in the EA group were significantly lower than those of mice in the model group during the same period(all P<0.05),and the scores of mice in the EA group were significantly lower than those in the sham EA group on days 12 and 14 of the experiment(all P<0.05).On days 8,10,12 and 14 of the experiment,the hindfoot 50%withdrawal thresholds of mice in the model group were significantly lower than those of mice in the control group(all P<0.05),and the latency period of hindfoot withdrawal of thermal pain was significantly shorter than that in the control group(P<0.05);on days 10,12 and 14 of the experiment,the hindfoot 50%with

关 键 词：偏头痛 硝酸甘油 电针 三叉神经血管系统 

分 类 号：R-332[医药卫生]