Nicastrin、N1ICD及hes1蛋白在小鼠肝脏及肝癌组织中的表达  

Expression of nicastrin,N1ICD and hes1 proteins in normal and cancerous liver tissues of mice

作　　者：严璐 史天威 YAN Lu;SHI Tianwei(The Fifth Clinical Medical College,He′nan University of Chinese Medicine(Zhengzhou People′s Hospital),Zhengzhou 450000,China;不详)

机构地区：[1]河南中医药大学第五临床医学院、河南中医药大学人民医院、郑州人民医院,郑州450000 [2]郑州大学人民医院、河南省人民医院、河南大学人民医院,郑州450003

出　　处：《实用医学杂志》2024年第6期768-772,共5页The Journal of Practical Medicine

基　　金：国家自然科学基金面上项目(编号:81773344);河南省医学科技攻关计划联合共建项目(编号:LHGJ20200685)。

摘　　要：目的 检测C57BL/6小鼠正常肝脏和肝癌组织中nicastrin、N1ICD和hes1蛋白的表达。方法 将12只6周龄雄性C57BL/6小鼠随机分为对照组和模型组,每组6只。模型组小鼠注射Hepa1-6肝癌细胞构建小鼠原位肝癌模型,对照组注射等量的生理盐水。HE染色观察肝脏癌变情况。IHC与Western blot检测nicastrin、N1ICD和hes1蛋白在正常肝脏组织及肝癌组织中的表达情况。结果 IHC显示,nicastrin、N1ICD及hes1蛋白在正常肝细胞中均定位于肝窦内皮细胞,肝细胞基本不表达;相比对照组肝脏组织,在模型组小鼠肝癌组织中,nicastrin蛋白在肝癌细胞中表达增多(P <0.01),N1ICD及hes1蛋白表达均减少(P <0.05,P <0.01)。结论 NCSTN基因在小鼠肝细胞癌中发挥促癌作用;notch1与hes1在小鼠肝细胞癌中可能发挥抑癌作用。Objective To detecting the expression of nicastrin,N1ICD and hes1 proteins in normal liver tissues and liver cancer tissues of C57BL/6 mice.Methods Twelve 6-week-old male C57BL/6 mice were ran-domly equally divided into the control group and model group.In the model group,the in situ liver cancer model was established with injection of Hepa1-6 cells into the liver,and the control group was treated with injection of the same amount of normal saline.Liver cancer was verified by HE staining.The expression of nicastrin,N1ICD and hes1 proteins in the normal and cancerous liver tissues was detected by IHC and Western blot.Results IHC results showed that nicastrin,N1ICD and hes1 proteins were localized in the hepatic sinusoidal endothelial cells in the normal hepatocytes,but not expressed in the hepatocytes.The model group showed higher expression of nicas-trin protein but lower expression of N1ICD and hes1 protein in the cancer cells compared with the control group.Conclusion NCSTN gene may play a carcinogenic role in mouse hepatocellular carcinoma,while notch1 and hes1 may play a carcinostasis role in the carcinoma.

关 键 词：NCSTN notch1受体 hes1蛋白 肝细胞癌 hepa1-6 小鼠 

分 类 号：R735.7[医药卫生—肿瘤]