糖尿病诱导的小鼠室旁核催产素和加压素阳性神经元FOS表达  

FOS expression in oxytocin and vasopressin positive neurons in paraventricular nucleus of mice induced by diabetes

作　　者：王淑敏 贾鹏 任淑婷 吕思婷 张婷 杨彦玲 史娟 WANG Shumin;JIA Peng;REN Shuting;LYU Siting;ZHANG Ting;YANG Yanling;SHI Juan(Department of Physiology,Medical School of Yan’an University,Yan’an 716000;Department of Human Anatomy,Histology and Embryology,Basic Medical College,Air Force Medical University,Xi’an 710032;Chinese People’s Liberation Army 94750 Military Hospital,Longyan 366200;Department of Anatamy,Medical School of Yan’an University,Yan’an 716000;Department of Pathogenic Biology,Medical School of Yan’an University,Yan’an 716000,China)

机构地区：[1]延安大学医学院生理学教研室,延安716000 [2]空军军医大学基础医学院人体解剖与组织胚胎学教研室,西安710032 [3]中国人民解放军94750部队医院,龙岩366200 [4]延安大学医学院解剖学教研室,延安716000 [5]延安大学医学院病原生物学教研室,延安716000

出　　处：《神经解剖学杂志》2024年第1期35-42,共8页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(82071253,31571188)。

摘　　要：目的:探索糖尿病(DM)不同状态下小鼠下丘脑室旁核(PVN)催产素和加压素阳性神经元的FOS表达变化。方法:腹腔注射溶媒或链脲佐菌素(STZ)制备对照组或糖尿病小鼠模型,根据机械痛测试区分糖尿病痛(DNP组)与非痛组(DWP组)。采用免疫组化和免疫荧光染色技术检测PVN内FOS、催产素(OXT)和加压素(VP)阳性神经元的分布及双标情况,并进行计数和比较。结果:7 d时三组(Control组、DNP组和DWP组)小鼠PVN内均有较多的FOS表达,而28 d时DWP和DNP组FOS的表达几近于无,与7 d组相比差异显著(P<0.05或0.001)。与对照组相比,DNP组和DWP组动物的VP和OXT荧光染色同样存在随着造模时间延长染色强度减弱的趋势(P<0.05)。VP、OXT与FOS的双标染色计数结果显示,在DWP 7 d组,VP/FOS双标细胞数为74.33±22.10,占VP阳性细胞数的(56.64±7.52)%,而OXT/FOS的双标率则仅为(10.44±3.14)%。在DNP 7 d组,OXT/FOS双标细胞数为51.00±31.80,占OXT阳性细胞数的(18.50±9.51)%,而VP/FOS的双标率仅为(9.34±3.27)%。与此相对,28 d组FOS的表达锐减,几乎无双标细胞。结论:下丘脑PVN内的VP和OXT阳性神经元在糖尿病痛与非痛,疾病发展的不同阶段存在明显不同的可塑性变化特点,理解这些变化对于揭示糖尿病及其并发症的神经机制具有重要意义。Objective:To explore the feature of FOS expression in oxytocin-and vasopressin-positive neurons in the hypothalamic paraventricular nucleus(PVN)under different status of diabetes mellitus(DM).Methods:Intraperitoneal injection of vehicle or STZ in mice was conducted to establish control or diabetes model.Mechanical sensitivity was evaluated by von Frey filament tests to distinguish diabetic neuropathic pain(DNP)from without-pain group(DWP).The expression of FOS,oxytocin(OXT)-and vasopressin(VP)-positive neurons,as well as their double labeling was detected by immunohistochemical and immunofluorescent staining.Cell counting and comparison were made in groups.Results:FOS expression was easily detected in the PVN in the three groups(Control group,DNP group and DWP group)at 7 days,while that in DWP and DNP groups at 28 days was hardly detectable,with the number being significantly different from the 7 days group(P<0.05 or 0.001).Likewise,compared with the control group,immunofluorescent signals for VP and OXT staining in the DNP and DWP groups also showed a trend of weakening as the modeling time increased(P<0.05).The cell counting after double staining for VP or OXT with FOS showed that,in the DWP group at 7 days,the number of VP and FOS double-labeled neurons was 74.33±22.10,accounting for(56.64±7.52)% of VP-positive cells,whereas the double labeling rate for OXT and FOS was only(10.44±3.14)%.In the DNP group at 7 days,the number of OXT and FOS double-labeled neurons was 51.00±31.80,accounting for(18.50±9.51)% of OXT-positive neurons,whereas the double labeling rate for VP and FOS was only(9.34±3.27)%.In contrast to these changes in 7 days group,the expression of FOS decreased sharply in the group of 28 days,thereby almost no double-labeled neurons.Conclusion:The plasticity changes of oxytocin-and vasopressin-positive neurons in the PVN are different depending on the status of pain and non-pain,and the stage of disease progression.Understanding the changes is of great significance for unravelling the neural me

关 键 词：糖尿病神经病理性痛 下丘脑室旁核 催产素 加压素 小鼠 

分 类 号：R747.9[医药卫生—神经病学与精神病学] R587.2[医药卫生—临床医学]