超高效液相色谱-串联质谱法测定水产品中5种卡因类麻醉剂及其代谢物的不确定度  

Uncertainty evaluation for determination of 5 caine anesthetics and their metabolites in aquatic products by ultra high performance liquid chromatography-tandem mass spectrometry

作　　者：肖璇 周凌聿 陈春泉 高云慨 万娜 尹青春 XIAO Xuan;ZHOU Lingyu;CHEN Chunquan;GAO Yunkai;WAN Na;YIN Qingchun(Institute of Food Testing,Hainan Academy of Inspection and Testing,Haikou 570314,China)

机构地区：[1]海南省检验检测研究院食品检验检测中心,国家市场监管重点实验室,海口570314

出　　处：《化学分析计量》2024年第3期121-128,共8页Chemical Analysis And Meterage

基　　金：海南省自然科学基金项目(321MS104)。

摘　　要：采用超高效液相色谱-串联质谱法,对水产品中卡因类麻醉剂3-氨基苯甲酸乙酯甲磺酸盐及其代谢物间氨基苯甲酸、苯佐卡因及其代谢物对氨基苯甲酸和对乙酰氨基苯甲酸残留量的不确定度进行评定,根据BJS 202110《水产品及相关用水中12种卡因类麻醉剂及其代谢物的测定》建立数学模型,分析影响测量不确定度的主要因素。目标水产品中3-氨基苯甲酸乙酯甲磺酸盐、间氨基苯甲酸、苯佐卡因、对氨基苯甲酸、对乙酰氨基苯甲酸质量分数测定结果分别为0.70、5.53、0.67、6.19、7.33μg/kg,扩展不确定度分别为0.07、0.52、0.09、0.59、0.99μg/kg(k=2,置信区间=95%)。结果显示该方法测量不确定度主要来源于标准溶液配制和标准曲线的拟合。该评定方法为卡因类麻醉剂的测定提供技术依据,解决该方法不确定度的溯源问题。The uncertainty of residues of 3-aminobenzoate and its metabolite m-aminobenzoic acid,benzocaine and its metabolite p-aminobenzoic acid and p-acetylaminobenzoic acid in aquatic products was evaluated by ultra high performance liquid chromatoc-tandem mass spectrometry.According to BJS202110 standard"Determination of 12 caine anesthetics and their metabolites in aquatic products and related water",a mathematical model was established to analyze the main factors affecting the measurement uncertainty.The calculated mass fraction of 3-aminobenzoate,m-aminobenzoic acid,benzocaine,p-aminobenzoic acid and p-acetylaminobenzoic acid in target aquatic products was 0.70,5.53,0.67,6.19 and 7.33μg/kg,respectively.The extended uncertainties were 0.07,0.52,0.09,0.59,0.99μg/kg(k=2,confidence interval=95%),respectively.The measurement uncertainty of this method mainly comes from the preparation of standard solution and the fitting of standard curve.This evaluation method provides technical basis for the determination of caine anesthetics and solves the problem of tracing the uncertainty of this method.

关 键 词：超高效液相色谱-串联质谱法 麻醉剂 水产品 3-氨基苯甲酸乙酯甲磺酸盐 苯佐卡因 不确定度 

分 类 号：O657.7[理学—分析化学]