红外心率测定技术在香港牡蛎保活运输过程中的应用  

Application of infrared heart rate measurement technology in the preservation and transportation of Crassostrea hongkongensis

作　　者：邓杰 常向阳 林恒宗 范秀萍[1,2] 秦小明 DENG Jie;CHANG Xiangyang;LIN Hengzong;FAN Xiuping;QIN Xiaoming(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,Guangdong,China;National Research and Development Branch Center for Shellfish Processing(Zhanjiang)Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety/Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution,Zhanjiang 524088,Guangdong,China)

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]国家贝类加工技术研发分中心(湛江),广东省水产品加工与安全重点实验室,水产品深加工广东普通高校重点实验室,广东湛江524088

出　　处：《渔业现代化》2024年第2期93-101,共9页Fishery Modernization

基　　金：财政部和农业农村部国家现代农业产业技术体系资助(贝类,CARS-49)。

摘　　要：心率能反映牡蛎活力水平和生理状态,可作为生命特征指标,用于监测牡蛎的生理代谢水平,本研究为优化香港牡蛎(Crassostrea hongkongensis)保活流通过程中心率测定技术,探究牡蛎保活期间心率的变化,为牡蛎活体流通提供理论依据。以香港牡蛎为研究对象,依据线性数学模型确定牡蛎心脏位置,并基于红外传感器结合PowerLab装置优化红外心率测定技术,探讨了牡蛎活体流通过程暂养盐度波动、线性诱导休眠和贮藏期中对牡蛎心率的影响。结果显示:经6 mm钻孔前处理后,在海水中暂养对牡蛎活力影响较小,第9天存活率仍达100%,且红外测定方法的第2~18 h内心率无显著性差异(P>0.05);个体规格、干露、盐度、温度均显著影响牡蛎的心率(P<0.05),海水贮藏牡蛎经红外技术测定的心率随时间呈显著上升趋势(P<0.05),干露贮藏牡蛎末期心率随时间呈先上升后下降(P<0.05),过度的非生物胁迫时会引起心脏停止搏动现象的产生,可能会对其造成不良影响。研究表明,红外测定技术适用于作为测定牡蛎心率技术,在保活期中心率变化有效反映了牡蛎的生理状态,可采用心率作为牡蛎无水保活的活力测定指标,对研究基于心率为指示牡蛎保活监测体系具有一定参考意义。Heart rate can reflect the vitality level and physiological status of oysters and can be used as a vital characteristic indicator to monitor the physiological metabolism level of oysters.This study aims to optimize the heart rate measurement technology during the survival and circulation process of Crassostrea hongkongensis and explore the survival of oysters.Taking C.hongkongensis as the research object,a linear mathematical model was used to determine the heart position of the oysters and the infrared heart rate measurement technology was optimized by combining infrared sensors and PowerLab devices.The effects of salinity fluctuations,linear induced dormancy,and storage period of oysters during the live circulation process were discussed.Effect of heart rate.Results:After pre-treatment with 6 mm drilling holes,temporary cultivation in seawater had little effect on the vitality of oysters.The survival rate still reached 100% on the 9th day.There was no significant difference in heart rate between 2 and 18 hours using the infrared measurement method(P>0.05);individual size,dry dew,salinity,and temperature all significantly affect the heart rate of oysters(P<0.05).The heart rate of oysters stored in seawater measured by infrared technology shows a significant upward trend over time(P<0.05).The heart rate of oysters exposed to storage at the end of the period increased and then decreased over time(P<0.05).Excessive abiotic stress can cause cardiac arrest,which may have adverse effects.Conclusion:Infrared measurement technology is suitable for measuring oyster heart rate.Changes in heart rate during the survival period effectively reflect the physiological state of oysters.Heart rate can be used as a vitality measurement indicator for oysters to stay alive without water.Research on the oyster survival monitoring system based on heart rate as an indicator has a certain reference significance.

关 键 词：香港牡蛎 心脏位置 优化技术 心率变化 活力指标 

分 类 号：S983[农业科学—捕捞与储运]