响应面法优化提取五指毛桃总香豆素及其抗氧化活性研究  

Extraction process optimization of total coumarins by response surface methodology from Ficus hirta and evaluation of their antioxidant activity

作　　者：王丹华 汤邝杰 曾雷[1] 王志宏 王颂 周玲艳 徐巧林[1] WANG Danhua;TANG Kuangjie;ZENG Lei;WANG Zhihong;WANG Song;ZHOU Lingyan;XU Qiaolin(Guangdong Academy of Forestry\Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangzhou 510520,China;College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)

机构地区：[1]广东省林业科学研究院\广东省森林培育与保护利用重点实验室,广东广州510520 [2]仲恺农业工程学院农业与生物学院,广东广州510225

出　　处：《仲恺农业工程学院学报》2023年第4期45-51,共7页Journal of Zhongkai University of Agriculture and Engineering

基　　金：广东省林业科技计划项目(2021KYXM01);广东省林业科技创新项目(2021KJCX004、2020KJCX013)。

摘　　要：为优化五指毛桃(Ficus hirta)总香豆素的提取工艺并明确其体外抗氧化活性,本研究以五指毛桃总香豆素得率为评价标准,通过单因素试验考察了乙醇体积分数、液料比以及超声时间对五指毛桃总香豆素得率的影响.通过Box-Behnken试验设计对五指毛桃总香豆素超声波辅助提取工艺进行优化,并对工艺优化后提取的总香豆素进行体外抗氧化活性研究.结果表明,五指毛桃总香豆素的最佳提取工艺为乙醇体积分数58%、液料比34∶1(mL/g)、超声时间70 min,在该条件下五指毛桃总香豆素平均得率为3.869 mg/g,相对标准偏差(Relative Standard Deviation,RSD)为0.28%,与预测值3.884 mg/g基本一致.体外抗氧化试验表明,五指毛桃总香豆素具有显著的抗氧化活性,对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhdrazyl,DPPH)、2,2′-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐(2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid),ABTS)自由基的半数抑制浓度(IC50)分别为2.23 mg/mL、0.33 mg/mL.采用响应面法优化的五指毛桃总香豆素提取工艺简便、可行,提取的五指毛桃总香豆素具有较强的抗氧化活性,可作为天然抗氧化剂.In order to optimize the extraction process of total coumarins from Ficus hirta and clarify its in vitro antioxidant activity,the extraction rate of total coumarins was used as the evaluation standard to investigate the effects of ethanol volume fraction,liquid material ratio and ultrasonic time on the extraction rate of total coumarins from the root of F.hirta by single factor experiment.The ultrasonic-assisted extraction process of total coumarins from the root of F.hirta was optimized by Box-Behnken experimental design,and the in vitro antioxidant activity of the total coumarins extracted by the optimized extraction process was studied.The results showed that the optimum extraction conditions were as follows:ethanol volume fraction 58%,liquid-solid ratio 34∶1(mg/mL),ultrasonic extraction time 70 min.The average extraction yield of total coumarins from the root of F.hirta was 3.869 mg/g under these conditions,with the relative standard deviation(RSD)was 0.28%,which was basically consistent with the predicted value of 3.884 mg/g.The in vitro antioxidant activity test results showed that the total coumarins from F.hirta have significant antioxidant activity.The median inhibition concentration(IC 50)values of total coumarins from the root of F.hirta were 2.23 mg/mL and 0.33 mg/mL for 1,1-diphenyl-2-picrylhdrazyl(DPPH)free radicals and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid)(ABTS)free radicals,respectively.The extraction process optimized by response surface methodology is simple and feasible.The total coumarins extracted from the root of F.hirta has strong antioxidant activity and can be used as a natural antioxidant source for further development and comprehensive application.

关 键 词：五指毛桃(Ficus hirta) 总香豆素 响应面法 超声波辅助 抗氧化活性 

分 类 号：S718.45[农业科学—林学]