草莓灰霉病病原菌分离鉴定及拮抗微生物筛选  

Isolation and Identification of Strawberry Gray Mold Pathogen and Screening of Antagonistic Microorganisms

作　　者：严浩 于佳乐 朱强 张悦 李安妮 李玉婷 夏艳秋 YAN Hao;YU Jiale;ZHU Qiang;ZHANG Yue;LI Anni;LI Yuting;XIA Yanqiu(School of Food Science and Engineering,Jiangsu Ocean University,Lianyungang 222005,China)

机构地区：[1]江苏海洋大学食品科学与工程学院,江苏连云港222005

出　　处：《江苏海洋大学学报（自然科学版）》2024年第1期16-23,共8页Journal of Jiangsu Ocean University:Natural Science Edition

基　　金：江苏省政策引导类计划苏北科技专项(LYG-SZ201806);连云港市“521工程”专项(LYG52105-2018046)。

摘　　要：草莓灰霉病是草莓种植过程中的主要病害之一。通过对连云港市东海县和徐州市贾汪区草莓基地草莓灰霉病病原菌进行分离纯化,获得一株生长优势病原菌,命名为A03。经过形态学观察、分子生物学鉴定,鉴定其为灰葡萄孢属(Botrytis cinerea)富克葡萄孢盘菌(Botryotinia fuckeliana)。利用平板对峙生长法筛选出一株对A03具有拮抗作用的微生物,命名为ZXY-1,通过分子生物学鉴定为海洋解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。ZXY-1对A03抑菌圈半径达12.67 mm, ZXY-1发酵上清液对A03孢子萌发相对抑制率为58.92%,结果显示ZXY-1发酵液对草莓果具有明显的保鲜作用。海洋解淀粉芽孢杆菌ZXY-1在草莓种植和生长中具备应用潜力。Strawberry gray mold is one of the important diseases in strawberry production.By isolating and purifying the pathogen of strawberry gray mold from strawberry bases in Donghai County,Lianyungang and Jiawang District,Xuzhou,a growth dominant pathogen A03 was obtained.After the morphological observation and molecular biological identification,it was identified as Botryotinia fuckeliana.A strain of ZXY-1 which had antagonistic effect on A03 was screened by plate confrontative growth method and identified as marine Bacillus amyloliquefaciens by molecular biology.ZXY-1 had inhibition radius of 12.67 mm on A03,and inhibition rate of ZXY-1 fermentation liquid on spore germination of A03 was 58.92%.The experiment showed that ZXY-1 fermentation liquid had obvious fresh-keeping effect on strawberry fruit.Therefore,marine Bacillus amyloliquefaciens ZXY-1 has the potential to be used in strawberry production.

关 键 词：草莓灰霉病 拮抗菌 解淀粉芽孢杆菌 抑菌活性 

分 类 号：Q939.95[生物学—微生物学]