miR-654-5p与ATM蛋白在食管癌中的表达及临床意义  

Expression and clinical significance of miR-654-5p and ATM protein in esophageal carcinoma

作　　者：韩笑[1] 朱平 陆荣柱 HAN Xiao;ZHU Ping;LU Rong-Zhu(Department of Pathology,Huaian Cancer Hospital Huaian Hospital,Huaian 223200,Jiangsu,China)

机构地区：[1]淮安市肿瘤医院淮安医院病理科,江苏淮安223200 [2]淮安市肿瘤医院淮安医院内分泌科,江苏淮安223200 [3]江苏大学医学院预防医学与卫生检验系

出　　处：《吉林医学》2024年第4期763-766,共4页Jilin Medical Journal

基　　金：江苏省卫生健康委员会项目[项目编号:LKM2022071];淮安市科技计划项目[项目编号:HAB202247]。

摘　　要：目的:探讨miR-654-5p与共济失调性毛细血管扩张症突变(ATM)蛋白在食管癌组织与癌旁切缘正常鳞状上皮组织中的表达及两者在食管癌发生中的作用及相关性。方法:采用荧光定量PCR检测miR-654-5p在186例食管癌及其癌旁正常食管黏膜组织中的表达;采用免疫组化SP法检测ATM蛋白在186例食管癌及其癌旁正常食管黏膜组织中的表达。结果:通过分析186例食管癌患者的临床病理特征,发现miR-654-5p在食管癌组织中高表达率,随着分化程度的降低、浸润深度的加深其高表达率增高;ATM蛋白阳性表达率随着分化程度的降低、浸润深度的加深而降低。两者在食管癌中表达与性别、年龄、肿块大小、肿块部位无相关性(P>0.05)。结论:miR-654-5p和ATM蛋白的表达与食管癌分化程度、浸润深度有关(P<0.05),两者在食管癌中的表达趋势相反(P=0.014),显示两者在食管癌的发生发展中有一定作用,且可能共同作用于食管癌的发生。Objective To investigate the expression of miR-654-5p and ataxic telangiectasia mutant protein ATM in esophageal carcinoma tissues and normal squamous epithelium adjacent to carcinoma,and to explore their roles and correlation in the development of esophageal carcinoma.Method Fluorescence quantitative PCR was used to detect the expression of miR-654-5p in 186 cases of esophageal cancer and adjacent normal esophageal mucosa.Immunohistochemical SP method was used to detect the expression of ATM protein in 186 cases of esophageal carcinoma and adjacent normal esophageal mucosa.Results By analyzing the clinicopathologic features of 186 patients with esophageal cancer,it was found that the high expression rate of miR-654-5p increased with the decrease of differentiation and the deepening of infiltration depth;The positive expression rate of ATM protein decreased with the decrease of differentiation degree and the deepening of infiltration depth.There was no correlation between the expression of both of them in esophageal cancer and gender,age,mass size and mass site(P>0.05).Conclusion The expression of miR-654-5p and ATM protein was related to the differentiation degree and infiltration depth of esophageal cancer(P<0.05),and the expression trend of miR-654-5p and ATM protein in esophageal cancer was opposite,the difference was statistically significant(P=0.014),indicating that the two proteins played certain role in the occurrence and development of esophageal cancer,and may jointly act on the occurrence of esophageal cancer.

关 键 词：食管癌 miR-654-5p ATM蛋白 荧光定量PCR 免疫组织化学 

分 类 号：R571[医药卫生—消化系统]