共刺激分子B7-H6在胃癌中的表达与胃癌临床病理特征之间的关系分析  

Expression of Co-stimulatory Molecule B7-H6 in Gastric Cancer and its Relationship with Clinicopathological Characteristics

作　　者：吴滨 赵鑫[2] 徐峰 WU Bin;ZHAO Xin;XU Feng(Department of Internal Medicine,Suzhou Industrial Park Xingpu Hospital,Suzhou,Jiangsu,215126,China;Department of Gastrointestinal Surgery,First Affiliated Hospital of Suchow University,Suzhou,Jiangsu,215006,China)

机构地区：[1]苏州工业园区星浦医院内科,江苏苏州215126 [2]苏州大学附属第一医院胃肠外科,江苏苏州215006

出　　处：《中国血液流变学杂志》2023年第4期500-506,577,共8页Chinese Journal of Hemorheology

基　　金：国家自然科学基金资助项目(82073180)。

摘　　要：目的研究胃癌组织中共刺激分子B7-H6的表达与临床病理特征之间的关系。方法TCGA数据库中搜集相关病例进行初步分析B7-H6在肿瘤组织以及正常组织中的表达情况,搜集苏州大学附属第一医院胃癌48例,免疫组化和RT-qPCR分别检测患者癌组织和正常组织中B7-H6的蛋白和mRNA表达水平。统计学分析B7-H6的表达水平与胃癌患者的年龄、性别、肿瘤位置、分化程度、分期、是否伴有病理学上的癌栓累及、神经侵犯等临床病理特征之间的相关性。结果TCGA数据结果显示B7-H6在胃癌肿瘤组织中的表达远高于正常组织中,并且差异具有统计学意义(P<0.0001);48例胃癌组织中B7-H6蛋白的阳性表达率明显高于正常组织(62.5%vs.39.6%,P=0.025);胃癌组织B7-H6 mRNA的表达水平高于正常组织(P<0.001)。B7-H6的表达与患者肿瘤分期、浸润深度及神经侵犯有关(P<0.05),而与年龄、性别、肿瘤位置、淋巴结转移情况、脉管内癌栓、分化程度等无相关性(P>0.05)。并且肿瘤分期越晚(P=0.004)、浸润深度越深(P=0.022)、伴有病理学上的神经侵犯时(P=0.004),B7-H6 mRNA的相对表达量越高。结论B7-H6的表达与患者肿瘤分期、浸润深度及神经侵犯有关,可能在胃癌的侵袭和增殖上具有促进作用。Objective To investigate the relationship between the expression of co-stimulatory molecule B7-H6 and clinicopathological features in gastric cancer tissues.Methods Relevant cases were collected from the TCGA database,and the expression of this factor in tumor tissues and normal tissues was preliminary analyzed,48 cases of gastric cancer from the First Affiliated Hospital of Soochow University were collected,and the protein and mRNA expression levels of B7-H6 in cancer tissues and normal tissues of patients were detected using immunohistochemistry and RT-qPCR,respectively.The correlation between the expression level of B7-H6 and clinical pathological characteristics such as age,gender,tumor location,differentiation degree,staging,presence of pathological cancer emboli,and nerve invasion in gastric cancer patients was analyzed using statistical methods.Results TCGA data showed that the expression of B7-H6 in gastric cancer tumor tissues was significantly higher than in normal tissues and was statistically significant(P<0.0001).The positive expression rate of B7-H6 protein in the 48 cases of gastric cancer tissue was significantly higher than in normal tissue(62.5%vs.39.6%,P=0.025),and the expression level of B7-H6 mRNA in gastric cancer tissue was higher than in normal tissue(P<0.001).The expression of B7-H6 was related to tumor staging,depth of infiltration,and nerve invasion(P<0.05),while it showed no correlation with age,gender,tumor location,lymph node metastasis,intravascular cancer emboli,and differentiation degree(P>0.05).Furthermore,the later the tumor staging(P=0.004),the deeper the infiltration(P=0.022),and the presence of pathological nerve invasion(P=0.004),the higher the relative expression level of B7-H6 mRNA.Conclusion The expression of B7-H6 is associated with tumor staging,depth of infiltration,and nerve invasion,which suggests a potential promoting role in the invasion and proliferation of gastric cancer.

关 键 词：TCGA B7-H6 RT-QPCR 胃癌 免疫组化 

分 类 号：R735.2[医药卫生—肿瘤]