腹腔镜胃癌根治性切除标本cyclin D1和SLeX表达的临床病理研究  

Clinicopathologic Study on Expression of Cyclin D1 and SLeX in Gastric Carcinoma Radically Resected by Laparoscopic Surgery

作　　者：左利媛 刘凌云 尚培中[1] 苗建军[1] 张伟[1] 王丽娜[1] 李伟[3] ZUO Li-yuan;LIU Ling-yun;SHANG Pei-zhong;MIAO Jian-jun;ZHANG Wei;WANG Li-na;LI Wei(Department of General Surgery,The 81 st Group Army Hospital of the PLA,Zhangjiakou,Hebei 075000,China;Integrated Department,Huailai Shiji Hospital,Huailai,Hebei 075400,China;Department of Pathology&Medical Laboratory,The 81 st Group Army Hospital of the PLA,Zhangjiakou,Hebei 075000,China)

机构地区：[1]中国人民解放军陆军第八十一集团军医院普通外科,河北张家口075000 [2]怀来世济医院综合科,河北怀来075400 [3]中国人民解放军陆军第八十一集团军医院检验病理科,河北张家口075000

出　　处：《河北北方学院学报（自然科学版）》2024年第6期10-13,16,共5页Journal of Hebei North University:Natural Science Edition

基　　金：张家口市大健康和生物医疗专项重点研发计划项目(No.2121129D)。

摘　　要：目的探讨腹腔镜胃癌根治性切除标本cyclin D1和SLeX表达与患者临床病理特征及预后的关系。方法85例腹腔镜胃癌根治性手术切除标本及30例癌旁胃组织,应用免疫组织化学染色EliVision^(TM)plus二步法检测cyclin D1和SLeX在胃癌及癌旁胃组织中的表达,分析cyclin D1和SLeX与患者性别、癌灶大小、分化程度、临床分期、转移浸润及5年生存期6项临床病理特征的相关性。结果癌组织cyclin D1和SLeX阳性率分别为61.2%(52/85)和85.9%(73/85),癌旁胃组织cyclin D1和SLeX阳性率分别为0和6.7%(2/30),差异均有统计学意义(P<0.05)。Cyclin D1和SLeX表达与性别及癌灶大小无关(P>0.05),在低分化、进展期、有转移浸润和5年内死亡患者中,Cyclin D1和SLeX阳性率均显著高于(高+中)分化、早期、无转移浸润和5年内生存患者(P<0.05)。Cyclin D1和SLeX表达呈正相关(r=0.40)。结论Cyclin D1和SLeX与胃癌发生发展显著相关,两者共阳性表达预示胃癌分化程度低、进展快、易浸润转移、生存期短。Objective To investigate the cyclin D1 and SLeX expression in gastric carcinoma radically resected by laparoscopic surgery and the relationship with clinicopathologic features and prognosis.Methods A retrospective study of 85 patients with gastric carcinoma who underwent laparoscopic resection was conducted.The expression of cyclin D1 and SLeX in 85 cancer tissues and 30 paracancerous tissues were detected by immunohistochemical EliVision~(TM) plus two-step method.The relationships between the expression of cyclin D1 and SLeX and the clinicopathologic features including patient gender,tumor size,differentiated degree,clinical stage,metastasis and(or)invasion,five-year survival rate were studied.Results The positive rates of cyclin D1 and SLeX were 61.2% and 85.9% for cancer tissues and 0 and 6.7% for paracancerous tissues,respectively,with statistically significant differences(P<0.05).There was no significant difference in the expression of cyclin D1 and SLeX between the gender and tumor size of two groups(P>0.05).In patients with poor differentiation,progressive stage,presence of metastasis and(or)invasion,death within 5 years of follow-up,the positive expression rate of cyclin D1 and SLeX were significantly higher than those in patients with high and moderate differentiation,early stage,absence of metastasis and(or)invasion,survival within 5 years of follow-up(P<0.05).A significant positive relationship was observed between Cyclin D1 and SLeX(r=0.40).Conclusion Both expression of cyclin D1 and SLeX significantly take part in the progress of gastric carcinoma.The Co-positive expression of cyclin D1 and SLeX may indicate poor differentiation,rapid progression,higher metastatic and(or)invasive potential and short survival period of cancer tissue.

关 键 词：胃癌 Cyclin D1 SLEX 肿瘤转移 肿瘤浸润 预后 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]