BMSC外泌体抑制Rho/ROCK信号通路对口腔癌细胞生物活性的作用机制  

The mechanism of BMSC Exosomes inhibiting Rho/ROCK signaling pathway on the biological activity of oral cancer cells

作　　者：赵倩 王继明[1] 易华 ZHAO Qian;WANG Ji-ming;YI Hua(Affiliated Hospital of Inner Mongolia Medical University,Inner Mongolia Hohhot 010010,China)

机构地区：[1]内蒙古医科大学附属医院,内蒙古呼和浩特010010

出　　处：《临床口腔医学杂志》2024年第3期140-144,共5页Journal of Clinical Stomatology

摘　　要：目的:探讨骨髓间充质干细胞(bone marrow mesenchymal stem cell,BMSC)外泌体(Exosomes)抑制Rho/ROCK信号通路对口腔癌细胞生物活性的作用机制。方法:细胞分组包括,对照组:正常胎牛血清培养Tca8113细胞;Exosomes组:Tca8113细胞与Exosomes标记的1μmol/L Dil共培养;Y27632组:Tca8113细胞与Rho/ROCK信号通路抑制剂Y27632共培养;联合组:Tca8113细胞+Exosomes+Y27632共培养。分别采用CCK-8检测细胞活性;流式细胞仪检测细胞凋亡率;划痕实验检测细胞迁移距离;Transwell法检测细胞侵袭数目;免疫印迹检测各组细胞RhoA、RhoC、ROCK1及ROCK2表达。结果:与对照组相比,Exosomes组24、48、72及96 h均降低,凋亡率升高,迁移距离及细胞侵袭数目均降低,Exosomes组、Y27632组比较差异无统计学意义;与Y27632组相比,联合组细胞活性、细胞迁移距离及侵袭数目均降低(P<0.05);与对照组相比,Exosomes组Tca8113细胞中RhoA、RhoC、ROCK1及ROCK2均降低(P<0.05);与Y27632组相比,联合组RhoA、RhoC、ROCK1及ROCK2均降低(P<0.05)。结论:BMSC外泌体(Exosomes)对口腔癌Tca8113细胞具有增加凋亡,抑制细胞活性、侵袭及迁移,研究机制可能与抑制Rho/ROCK信号通路激活相关。Objective:To investigate the mechanism of inhibition of Rho/ROCK signal pathway by Exosomes of bone marrow mesenchymal stem cell(BMSC) on the biological activity of oral cancer cells.Methods:Cell groups included control group:normal fetal bovine serum cultured Tca8113 cells.In the Exosomes group,Tca8113 cells were co-cultured with 1 μmol/L Dil labeled with Exosomes.Y27632 group:Tca8113 cells were co-cultured with Rho/ROCK signaling pathway inhibitor Y27632.Combination group:Tca8113 cells were co-cultured with Exosomes+Y27632.The cell activity was detected by CCK-8.The apoptosis rate was detected by flow cytometry.Cell migration distance was detected by scratch test.The number of cell invasion was detected by Transwell method.The expression of RhoA,RhoC,ROCK1 and ROCK2 were detected by Western blot.Results:Compared with the control group,both the apoptosis rate and migration distance and the number of cell invasion are decreased at 24,48,72,and 96 hours,with no statistically significant difference between the Exosomes group and Y27632 group.Compared with Y27632 group,the cell activity,cell migration distance and invasion number in combination group were decreased(P<0.05).Compared with the control group,RhoA,RhoC,ROCK1,and ROCK2 in Tca8113 cells were decreased in Exosomes group(P<0.05).Compared with Y27632 group,RhoA,RhoC,ROCK1 and ROCK2 in combination group were decreased(P<0.05).Conclusion:Exosomes of BMSC can increase apoptosis,inhibit cell activity,invasion and migration of oral cancer Tca8113 cells.The mechanism may be related to inhibition of Rho/ROCK signal pathway activation.

关 键 词：口腔癌 外泌体 增殖 凋亡 Rho/ROCK 

分 类 号：R739.8[医药卫生—肿瘤]