Intervention effect of Cigu Xiaozhi prescription(慈菇消脂方)on ceramide lipoapoptosis in non-alcoholic fatty liver disease  

作　　者：YANG Shaojun MA Yanhua BAI Zhouxia YU Ye FANG Buwu ZHANG Li WANG Li 

机构地区：[1]Department of Spleen and Stomach Diseases,Beihai Traditional Chinese Medicine hospital,Beihai 536000,China [2]the First Clinical Medical College of Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China [3]Department of Clinical Laboratory,Affiliated Hospital of Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China [4]Department of Pharmacology,School of Basic Medicine,Tianjin Medical University,Tianjin 300070,China

出　　处：《Journal of Traditional Chinese Medicine》2024年第1期63-69,共7页中医杂志（英文版）

基　　金：Natural Science Foundation-funded Project:the Interaction of mi RNAs and Hh Signaling Pathway in NASH Related Liver Fibrosis and the Intervention of Sagittaria Xiaozhi Pill(No.81860821);Toxin and Eliminating Phlegm Intervention Ceramide and Induced iNOS Nonalcoholic Fatty Liver Disease Fat Research of Apoptosis Signaling(No.81460710)。

摘　　要：OBJECTIVE:To explore the mechanism of the Chinese medicine Cigu Xiaozhi prescription(慈菇消脂方,CGXZ)in the treatment of the non-alcoholic fatty liver disease(NAFLD)by detoxification and phlegm-reducing,the effect of CGXZ prescription on ceramide-mediated lipid apoptosis in Hep G2 cells with NAFLD.METHODS:The experiment was randomly divided into 6groups:normal control group,model group,CGXZ prescription medicated serum high,medium,and low dose groups,and pioglitazone positive control group.Using 500μmol/L free fatty acid(FFA)mixture to induce Hep G2 cells to establish NAFLD cell model,respectively,with 2%,4%,and 6%concentration of CGXZ prescription medicated serum intervention for 24 h.The changes in organelles and lipid droplet accumulation were observed under the electron microscope.Furthermore,Td T-mediated d UTP Nick-End Labeling method was used to assay hepatocyte apoptosis;Biochemical determination of glutamic-pyruvic transaminase,glutamic oxalacetic transaminase,triglycerides,and FFA levels in Hep G2cells;the content of ceramide was determined by highperformance thin-layer chromatography.Finally,Western Blot and quantitative real-time polymerase chain reaction(q RT-PCR)were used to determine the protein and gene expression levels,such as inducible nitric oxide synthase(i NOS),nuclear factorκB(NF-κB),B cell lymphoma 2(Bcl-2)and Bcl-2-associated X(Bax).RESULTS:Under the electron microscope,the cells in the model group showed moderate-to-severe steatosis,and apoptotic bodies could be seen.The model group had greater improvements in the apoptosis rate(P<0.01),and the levels of ceramide C2 and FFA in the cytoplasm(P<0.01)than the normal control group.The protein expressions of NF-κB,i NOS,and Bax were significantly up-regulated(P<0.05),while the Bcl-2 had no significant change(P>0.05).Compared with the model group,the levels of ceramide C2 and FFA(P<0.01),the protein expressions of NF-κB,i NOS,and Bax(P<0.05)in the CGXZ prescription treatment group and pioglitazone positive control group were signific

关 键 词：non-alcoholic fatty liver disease CERAMIDES Cigu Xiaozhi prescription detoxification and phlegm removal 

分 类 号：R285[医药卫生—中药学]